Abstract: Objective To study the relation between the function of sperm membrane and seminal parameters. Methods Five hundred and three semen samples were collected from 149 normal men and 354 infertile men. Sperm concentration，motility，viability，Semen WBC concentration，morphology and sperm tail hypo-osmotic swelling (HOS) were analyzed according to WHO criteria. Results We observed sperm HOS score in infertile group was lower than that in control group（P<0.05）.Comparing with control group, the percentage of the concentration ,motility, viability ,as well as the percentage of normal morphology sperm, were significantly decreased in the normal and the abnormal HOS score infertile group, however, the rate of Head defects sperm and semen WBC concentration were significantly increased in these two groups（P<0.05）.In addition, the rate of Tail defects sperm in normal HOS score infertile group was higher than that in control group（P<0.05）.Comparing with abnormal HOS scores infertile group , sperm motility ,as well as sperm viability, was significantly decreased in normal HOS score infertile group（P<0.05）. Significant positive relationship of sperm HOS score was observed with sperm concentration ,motility and viability（P<0.05）.There was negative relationship between sperm HOS scores and semen WBC concentration（P<0.05）. Conclusion Our study shows that an important relationship can be found between the functional integrity of sperm membrane and semen parameters. Detection of sperm membrane function plays a key role in evaluation of male fertility.