大鼠MSCs诱导分化为心肌样细胞时钙、钾离子通道基因的表达

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (1): 36-39.

大鼠MSCs诱导分化为心肌样细胞时钙、钾离子通道基因的表达

王金菊田杰陈沅

重庆医科大学附属儿童医院心血管内科重庆医科大学儿科学院心内科重庆医科大学儿童医院心血管内科重庆

收稿日期:2005-12-08 修回日期:2006-06-30 出版日期:2007-01-25 发布日期:2007-01-25
通讯作者: 王金菊

Expression of ion channel gene after MSCs differentiating into cardiomyocytes

Received:2005-12-08 Revised:2006-06-30 Online:2007-01-25 Published:2007-01-25

摘要/Abstract

摘要： 目的研究大鼠骨髓间充质干细胞(MSCs)体外向心肌样细胞分化过程中L-型钙通道（α1C）和瞬间外向钾通道（kv4.3）基因的表达。方法无菌条件下冲洗Wistar大鼠双侧股骨和胫骨的骨髓腔获得MSCs，体外培养传代纯化，5-氮杂胞苷(5-aza)诱导24h，显微镜下观察诱导前后形态变化，RT-PCR鉴定α1C和kv4.3基因的表达。结果 kv4.3基因诱导前有弱的表达，诱导后1、4、7和14d表达明显逐渐增强(P<0.05)；α1C基因诱导前有较强的表达，诱导后1、4、7和14d表达却明显逐渐减弱(P<0.05)。结果 α1C和kv4.3基因在维持MSCs的诱导分化过程中发挥重要作用。

Abstract: Objective To study the expression of L-type calcium channel gene(α1c) and transient outward potassium channel gene (kv4.3 )during the procession of MSCs differentiating into cardiomyocytes. methods MSCs were isolated cultured,passaged, purified in vitro,and then exposed to 5-aza for 24 hours. we observed the morphology with microscopy. The expression ofα1C and kv4.3 gene was detected by Reverse transcription-polymerase chain reaction.Result:It displayed that MSCs had already expressed α1c and kv4.3.mRNA before 5-aza treatment. The expression of kv4.3 was significantly increased after 5-aza exposure at 24 hours ,4th day ,7th day,14th day,wheras the expression of α1C was impaired. Conlusion:L-type calcium channel and transient outward potassium channel played important roles during procession of MSCs differentiating into cardiomyocytes.

王金菊田杰陈沅. 大鼠MSCs诱导分化为心肌样细胞时钙、钾离子通道基因的表达[J]. 基础医学与临床, 2007, 27(1): 36-39.

. Expression of ion channel gene after MSCs differentiating into cardiomyocytes[J]. Basic & Clinical Medicine, 2007, 27(1): 36-39.