基础医学与临床 ›› 2022, Vol. 42 ›› Issue (11): 1731-1736.doi: 10.16352/j.issn.1001-6325.2022.11.1731

• 研究论文 • 上一篇    下一篇

土槿皮乙酸通过miR-4282抑制人弥漫大B细胞淋巴瘤细胞系SU-DHL-4的增殖

袁军1, 韩虎2, 董巍3, 王瑞仓1, 郝洪岭1*, 张学军4   

  1. 河北省人民医院 1.血液科; 2.急诊科,河北 石家庄 050055;
    3.石家庄市第三医院 心内科,河北 石家庄 050000;
    4.河北医科大学第二医院 血液内科,河北 石家庄 050055
  • 收稿日期:2021-11-18 修回日期:2022-07-31 出版日期:2022-11-05 发布日期:2022-11-01
  • 通讯作者: * qdl382@163.com
  • 基金资助:
    河北省医学科学研究课题计划(20190282)

Pseudolaric acid B inhibits the proliferation of human diffuse large B-cell lymphoma cell line SU-DHL-4 via miR-4282

YUAN Jun1, HAN Hu2, DONG Wei3, WANG Rui-cang1, HAO Hong-ling1*, ZHANG Xue-jun4   

  1. 1. Department of Hematology; 2. Department of Emergency, Hebei Provincial People's Hospital, Shijiazhuang 050055;
    3. Department of Cardiology, Shijiazhuang Third Hospital, Shijiazhuang 050000;
    4. Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang 050055, China
  • Received:2021-11-18 Revised:2022-07-31 Online:2022-11-05 Published:2022-11-01
  • Contact: * qdl382@163.com

摘要: 目的 观察土槿皮乙酸(PAB)通过调控miR-4282对人弥漫大B细胞淋巴瘤细胞系SU-DHL-4增殖和凋亡的影响。方法 将SU-DHL-4细胞分为对照组、PAB-L组、PAB-M组、PAB-H组。按照脂质体法将miR-NC、miR-4282转染至细胞,记为miR-NC组、miR-4282组;将anti-miR-NC、anti-miR-4282转染至细胞,用20 μmol/L PAB培养细胞,记为PAB+anti-miR-NC组、PAB+anti-miR-4282组。采用CCK8法检测细胞增殖;流式细胞测量术检测细胞凋亡;Western blot检测Ki-67、cleaved caspase-3、cleaved caspase-9蛋白表达;RT-qPCR检测miR-4282表达。结果 与对照组相比,PAB-L组、PAB-M组、PAB-H组Ki-67蛋白表达降低,miR-4282表达量及凋亡率、细胞增殖抑制率、蛋白cleaved caspase-3、cleaved caspase-9表达增加(P<0.05)。与miR-NC组相比,miR-4282组Ki-67蛋白表达降低,miR-4282表达量及凋亡率、细胞增殖抑制率、cleaved caspase-3、cleaved caspase-9表达增加(P<0.05)。抑制miR-4282表达可以降低PAB对SU-DHL-4细胞增殖和凋亡的影响。结论 土槿皮乙酸可能通过增加miR-4282抑制SU-DHL-4细胞增殖和促进凋亡。

关键词: 土槿皮乙酸, miR-4282, 弥漫大B细胞淋巴瘤, 增殖, 凋亡

Abstract: Objective To observe the effect of pseudolaric acid B(PAB) on the proliferation and apoptosis of human diffuse large B-cell lymphoma cell line SU-DHL-4 possibly and to explore the potential mechanism through regulating miR-4282. Methods SU-DHL-4 cells were divided into control group, PAB-L group, PAB-M group, PAB-H group; According to liposome method, miR-NC and miR-4282 were transfected into the cells and were marked as miR-NC group, miR-4282 group; The anti-miR-NC, anti-miR-4282 were transfected into the cells,cultured with 20 μmol/L PAB, denoted as (PAB+anti-miR-NC)group and(PAB+anti-miR-4282)group. Cell pro- liferation was detected by CCK8 assay, and cell apoptosis was detected by flow cytometry. The expression of Ki-67,cleaved caspase-3, cleaved caspase-9 protein was detected by Western blot and miR-4282 expression was detected by RT-qPCR. Results Compared with the control group, Ki-67 protein expression in PAB-L group, PAB-M group, and PAB-H group was decreased while miR-4282 expression, and the apoptosis rate, cell inhibition rate, protein expression of cleaved caspase-3, cleaved caspase-9 were increased (P<0.05). Compared with the miR-NC group, Ki-67 protein expression in miR-4282 group decreased, while miR-4282 expression, the apoptosis rate, cell inhibition rate, protein expression of cleaved caspase-3, cleaved caspase-9 were all increased (P<0.05). Inhibition of miR-4282 expression reduced the effect of PAB on the proliferation and apoptosis of SU-DHL-4 cells. Conclusions PAB may inhibit the proliferation of human diffuse large B-cell lymphoma cell SU-DHL-4 and promote apoptosis through increased expression of miR-4282.

Key words: pseudolaric acid B, miR-4282, diffuse large B-cell lymphoma, proliferation, apoptosis

中图分类号: