miR-760抑制人食管鳞状细胞癌细胞系的增殖、侵袭和迁移

doi:10.16352/j.issn.1001-6325.2022.11.1697

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (11): 1697-1703.doi: 10.16352/j.issn.1001-6325.2022.11.1697

miR-760抑制人食管鳞状细胞癌细胞系的增殖、侵袭和迁移

袁生武^1*, 陈娟², 刘凯¹

1.郑州大学第五附属医院介入科, 河南郑州 453000;
2.河南省人民医院超声诊断科, 河南郑州 453000

收稿日期:2021-10-14 修回日期:2022-04-07 出版日期:2022-11-05 发布日期:2022-11-01
通讯作者: ^* ygeeas@163.com
基金资助:
河南省医学科技攻关项目(LHGJ20190421)

miR-760 inhibits proliferation, invasion and migration of human esophageal squamous cell carcinoma cell lines

YUAN Sheng-wu^1*, CHEN Juan², LIU Kai¹

1. Department of Interventional Medicine, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 453000;
2. Department of Ultrasound Diagnosis, Henan Provincial People's Hospital, Zhengzhou 453000, China

Received:2021-10-14 Revised:2022-04-07 Online:2022-11-05 Published:2022-11-01
Contact: ^* ygeeas@163.com

摘要/Abstract

摘要： 目的探讨miR-760通过靶向调控碱性亮氨酸拉链ATF样转录因子3(BATF3)对食管鳞状细胞癌(ESCC)细胞系增殖、侵袭和迁移的影响。方法收集ESCC患者癌组织及其癌旁组织,体外培养人食管鳞状上皮细胞Het-1A和人ESCC细胞系EC9706、KYSE150、ECA109、TE-10。RT-qPCR检测miR-760和BATF3 mRNA表达。转染miR-760-mimics至TE-10细胞,上调miR-760表达,CCK-8法检测细胞增殖;Transwell小室检测细胞侵袭、迁移;Western blot检测增殖相关蛋白cyclin D1、p21及上皮-间质转化相关蛋白MMP2、vimentin、E-cadherin表达;双荧光素酶报告基因实验证实miR-760和BATF3的靶向关系。结果与癌旁组织和Het-1A细胞相比,ESCC组织和细胞系中miR-760表达水平明显降低,而BATF3表达水平明显升高(P＜0.05)。选择miR-760表达量最高的TE-10细胞进行后续实验。过表达miR-760可使TE-10细胞活性降低,侵袭、迁移细胞数减少,cyclin D1和vimentin蛋白表达降低,p21和E-cadherin蛋白表达升高(P＜0.05);miR-760负调控BATF3表达(P＜0.05);上调BATF3表达逆转了过表达miR-760对TE-10细胞增殖、侵袭、迁移的抑制作用(P＜0.05)。结论 miR-760过表达可抑制ESCC细胞的增殖、侵袭和迁移,其可能与靶向抑制BATF3表达有关。

关键词: miR-760, 碱性亮氨酸拉链ATF样转录因子3(BATF3), 食管鳞状细胞癌, 增殖, 迁移

Abstract: Objective To investigate the effects of miR-760 on the proliferation, invasion and migration of esophageal squamous cell carcinoma (ESCC) cells through targeted regulation of basic leucine zipper ATF-like transcription factor 3 (BATF3). Methods The cancer tissues and adjacent tissues of ESCC patients were collected, and normal human esophageal squamous epithelial cells Het-1A and human ESCC cell lines EC9706, KYSE150, ECA109, TE-10 were cultured in vitro. RT-qPCR was used to detect the expression of miR-760 and BATF3 mRNA. miR-760-mimics were transfected into TE-10 cells for up-regulating the expression of miR-760. CCK-8 method was used to detect cell proliferation and Transwell chamber was used to detect cell invasion as well as migration. Western blot was used to detect the expression of proliferation-related proteins cyclin D1, p21 and epithelial-mesenchymal transition related proteins MMP2, vimentin, and E-cadherin. Dual luciferase reporter gene experiment was used to confirm the targeting relationship between miR-760 and BATF3. Results Compared with the adjacent tissues and Het-1A cell, the expression of miR-760 was significantly reduced in ESCC tissues and in cell lines, while the expression of BATF3 increased (P＜0.05). The TE-10 cells with the highest expression of miR-760 were selected for subsequent experiments. Over-expression of miR-760 reduced the activity of TE-10 cells and the counting numbers of invasion and migration cells(P＜0.05). The expression of cyclin D1 and vimentin proteins increased the expression of p21 and E-cadherin proteins (P＜0.05). The results showed that miR-760 negatively regulated the expression of BATF3 (P＜0.05). Up-regulation of BATF3 expression reversed the inhibitory effects by over-expression of miR-760 on the proliferation, invasion and migration of TE-10 cells(P＜0.05). Conclusions Over-expression of miR-760 may inhibit the proliferation, invasion and migration of ESCC cells, which may be related to the targeted inhibition of BATF3 expression.

Key words: miR-760, basic leucine zipper ATF-like transcription factor 3(BATF3), esophageal squamous cell carcinoma, prolifera-tion, migration

中图分类号:

袁生武, 陈娟, 刘凯. miR-760抑制人食管鳞状细胞癌细胞系的增殖、侵袭和迁移[J]. 基础医学与临床, 2022, 42(11): 1697-1703.

YUAN Sheng-wu, CHEN Juan, LIU Kai. miR-760 inhibits proliferation, invasion and migration of human esophageal squamous cell carcinoma cell lines[J]. Basic & Clinical Medicine, 2022, 42(11): 1697-1703.

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miR-760抑制人食管鳞状细胞癌细胞系的增殖、侵袭和迁移

miR-760 inhibits proliferation, invasion and migration of human esophageal squamous cell carcinoma cell lines

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