HPLC Analysis of Monacolin J in Biosynthesis

Chinese Pharmaceutical Journal ›› 2012, Vol. 47 ›› Issue (7) : 543-546.

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PDF(978 KB)
Chinese Pharmaceutical Journal ›› 2012, Vol. 47 ›› Issue (7) : 543-546.

HPLC Analysis of Monacolin J in Biosynthesis

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Abstract

OBJECTIVE To establish an HPLC analysis method of monacolin J(MJ). METHODS MJ sample was analyzed with Hypersil C18 HPLC column. The mobile phase was composed of 0.1% H3PO4 and acetonitrile (30∶70) with flow rate of 1.0 mL·min-1, and the detection wavelength was 238 nm. Monacolin J samples produced from lovastatin fermentation mycelia and engineered Aspergilus terreus were analyzed. RESULTS The calibration curve of monacolin J was linear in the range of 10-500 μg·mL-1 with r of 0.999 8. The purity of crystaline monacolin J from lovastatin fermentation mycelia was 92%, and the purification yield was 96.24%. Meanwhile the engineered Aspergilus terreus produced lovastatin and monacolin J at the ratio of about 1.1∶1.0. CONCLUSION This HPLC method can separate lactone form and acid form of monacolin J, lovastatin and simvastatin very well. This HPLC method can be effectively used in monacolin J research of simvastatin biosynthesis.

Key words

monacolin J / simvastatin / lovastatin J / HPLC / biosynthesis

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HPLC Analysis of Monacolin J in Biosynthesis[J]. Chinese Pharmaceutical Journal, 2012, 47(7): 543-546

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[1] PLOSKER G L, MCTAVISH D. Simvastatin: a reappraisal of its pharmacology and therapeutic efficacy in hypercholesterolaemia [J]. Drugs, 1995,50:334-363.
[2] FRANS V D, MARIA L J, PHILOMINA V H, et al. Process for producing simvastatin and/or its derivatives [P]. WO:9945003,1999-09-10.
[3] KOBE N T, KAKOGAWA K I. Process for producing simvastatin [P]. US: 6331641, 2001-12-18.
[4] ZHU Z Y, WU T Z, MOU L. Synthesis of simvastatin [J]. Zhejiang Chemical Industry(浙江化工),2002,33(1):49-55.
[5] TANG Y, XIE X K. Methods and materials for making simvastatin and related compounds[P]. WO2007/139871A2,2007-06-12.
[6] YANG Z Y, GAN C H. Biosynthesis of simvastatin [J]. Chin J Biotechnol (生物工程学报),2008,24(3):349-354.
[7] JAEHEON L. Process for the preparation of simvastatin[P]. WO: 2003/057684,2003-07-17
[8] XIE X K, WATANABE K, WOJCICKI W A, et al. Biosynthesis of lovastatin analogs with a broadly specific acyltransferase [J]. Chem Biol, 2006,13:1161-1169.
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