Determination of the Activity of PEOZ Liposomal SOD Mimic Using NBT-Illumination Method
XU Huan1， YU Tao1， YIN Peng-peng1， ZHANG Wei1， WANG Kai-qian2， WANG Che1， YOU Zhong-lu1
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Received
Revised
Published
2011-12-22
2012-11-19
2012-11-16
Issue Date
2012-12-05
Abstract
OBJECTIVE To establish the method for determinating the activity of [poly(2-ethyl-2-oxazoline)， PEOZ]-modified liposomes encapsulated superoxide dismutase (SOD) mimic (PEOZ-L-SOD). METHODS PEOZ-L-SOD was prepared by film dispersion method， and the activities of the SOD mimic and PEOZ-L-SOD were determined by NBT-Illumination method. RESULTS The fitted equation of the inhibition rate curve of the SOD mimic was IR%=33.421lnρ+49.715（r2=0.999 2）and the IC50 was 1.008 6×10-3 μmol·L-1；the fitted equation of the inhibition rate curve of PEOZ-L-SOD was IR%=33.521lnρ+49.671（r2=0.999 1） and the IC50 was 1.009 9×10-3 μmol·L-1. CONCLUSION The NBT-Illumination method is simple， reliable， economic and practical， and can be used as an efficient method to determine the activity of SOD mimic liposomes. The experiment proves that the activity of SOD mimic did not change after it was encapsulated in the liposomes.
XU Huan， YU Tao， YIN Peng-peng， ZHANG Wei， WANG Kai-qian， WANG Che， YOU Zhong-lu.
Determination of the Activity of PEOZ Liposomal SOD Mimic Using NBT-Illumination Method[J]. Chinese Pharmaceutical Journal, 2012, 47(21): 1732-1735
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References
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