OBJECTIVE To investigate the effects of Buyanghuanwu decoction on preventing atherosclerosis induced by Hcy in rats in vivo. METHODS The model of atherosclerosis in rats was induced by intragastric gavage of Hcy. The intervention group was adminstered Buyanghuanwu decoction（5，10， 20 g·kg-1·d-1）. After 8 weeks treatment， the area of atherosclerotic plaques， blood vessel walls and their ratio， and ROS contention in the aorta were analyzed. The positive cell ratio of NF-κB P65 in vascular was detected by immunohistochemistry， and the expression of I-κBα was determined by Western-blotting. RESULTS The rats in the model group showed evident AS lesions which had significant difference with others groups. Compared with control group， the ROS content of model group was elevated (P<0.01)， the I-κBα protein in the vascular tissue was significantly reduced， and NF-κB was significantly activated. Different doses of Buyanghuanwutang decoction could decrease ROS content (P<0.01)， improve I-κBα protein and inhibit the activation of NF-κB. CONCLUSION Buyanghuanwutang decoction can prevent atherosclerosis induced by Hcy via inhibiting NF-κB-dependent pathway.
Key words
Buyanghuanwutang decoction /
homocysteine /
atherosclerosis /
reactive oxygen species /
NF-κB
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References
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