Study on Quality Control of Ranunculi Ternati Radix
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(Key Laboratory of Biomedical Engineering of Education，College of Biomedical Engineering and Instrument Science， Zhejiang University， Hangzhou 310027， China
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History+
Received
Revised
Published
2011-06-29
2011-06-29
2012-01-15
Issue Date
2012-04-14
Abstract
OBJECTIVE To identify a characteristic chemical marker of Ranunculi Ternati Radix and establish a HPLC method for determination of its content. METHODS Various chromatographic methods were applied to isolate the marker 4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl]butanoic acid， and its structure was elucidated by spectroscopic methods. The HPLC was performed on Agilent TC-C18 column（4.6 mm×250 mm，5 μm） using CH3OH-H2O (containing 0.4% acetic acid) as the mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 292 nm. RESULTS 4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl]butanoic acid was isolated and identified as the chemical marker. The HPLC method showed good linearity within the range of 0.372-1.302 μg (r2=0.999 8). The average recovery of 4-[formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl]butanoic acid was 100.89% (n=6，RSD=0.83%). CONCLUSION This method is simple， rapid and reliable for the quality control of Ranunculi Ternati Radix.
Study on Quality Control of Ranunculi Ternati Radix[J]. Chinese Pharmaceutical Journal, 2012, 47(2): 101-103
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References
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