OBJECTIVE A im To investigate the role of qercetin( Que)-induced autophagy in the death of hepatocellular carcinoma cell line SMMC-7721 cells. METHODS After treatment with different concentration of Que， the growth inhibition of the SMMC-7721 cells were assessed by MTT colorimetric assay. The fluorescent staining was applied to identify the autophagy after Que treatment. Lactate dehydrogenase (LDH) leakage and Western blot analysis were used to study the autophagic mechanisms involved in death of SMMC-7721 cells. RESULTS The proliferation of SMMC-7721 cells were significantly inhibited in a dose and time-dependent manner after Que treatment. Autophagy was also induced in MCF-7 cells as detected by MDC staining and Fluorescent staining in the early phase. The autophagy specific inhibitor 3-MA or chloroquine potentiated Que′s cytotoxicitiy in SMMC-7721 cells when administered 1h before Que； The expression of cathepsin B increased after Que treatmen. CONCLUSION Que can significantly inhibit the growth of the SMMC-7721 cells by inducing the autophagy， which is a protection mechanism that can reduce the cytotoxicity induced by Que in SMMC-7721 cells. The activation of Cathepsin B is considerable in autophagy process.
ZHU Mo-lia，b，LIANG Jin-yinga ， LI Penga， ZHAO Fan-ronga， ZHANG Yu-lina ， GAO Zhi-taoc*(a..
Effects of Autophagy on Quercetin-Induced Death of SMMC-7721 Cell[J]. Chinese Pharmaceutical Journal, 2012, 47(13): 1052-1055
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] DING W X， MANLEY S， MIN H. The emerging role of autophagy in alcoholic liver disease [J].Exp Biol Med， 2011， 236（5）：546-556.[2] LEE H M， SHI D M， YUK J M， et al. Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1 [J].J Immunol， 2011，186（2）：1248-1258.[3] NIU G M， YIN S M， XIE S F， et al. Quercetin induces apoptosis by activating caspase-3 and regulating Bcl-2 and cyclooxygenase-2 pathways in human HL-60 cells [J].Acta Biochim Biophys Sin，2011， 43（11）：30-37.[4] LU Y， DU X， LAI L， et al. Actinidia macrosperma C. F. Liang (a wild kiwi)：preliminary study of its antioxidant and cytotoxic activities [J].Evid Based Complement Alternat Med， 2012，2012：180262.[5] ZHU M L， HUA W M， LIANG Z Q. Relationsbetween autophagy and apoptosis on quercetin-induced death of human breast cancer cell line MCF-7 [J].Chin Pharm J(中国药学杂志)， 2010，45（6）：434-439.[6] ROSENFELDT M T， RYAN K M. The multiple roles of autophagy in cancer [J].Carcinogenesis， 2011， 32（7）：955-963. [7] VALENTOVIC M， BALL J G， BROWN J M. Cisplatin mediated alterations in oxidative stress enzymes are modulated by resveratrol [J].FASEB J， 2011，25（6）：1087.14.[8] GARCUA-MARCOS M， EAR M， FARQUHAR M G， et al. A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals [J].Mol Biol Cell， 2011，22（5）：673-686.[9] RONG Y G， MCPHEE C K， DENG S S， et al. Spinster is required for autophagic lysosome reformation and mTOR reactivation following starvation [J]. PNAS， 2011， 108（9）：7826-7831.[10] LE Q T， GOMEZ G， ZHAO W， et al. Processing of human protryptase in mast cells involves cathepsins L， B， and C [J].J Immunol， 2011， 187（4）：1912-1918.
