OBJECTIVE To establish a method for the determination of 19 carbamate pesticides in Chinese medicinal materials by UPLC triple-quadrupole tandem mass spectrometer. METHODS The samples were determinated by multi-reactions monitoring (MRM technology. The separation was performed on a Waters ACQUITY UPLC BEH C₁₈ column with acetonitrile -0.1% formic acid (1∶1 with a flow rate of 0.2 mL min-1. Ion mode was set as ESI+. RESULTS The standard curves of the 19 carbamate pesticides showed good linearity over the concentration range of 1.0-100 μg·L-1 （r=0.994 7-0.999 7， respectively. The LODs of 19 carbamate pesticides were in the range of 0.02-0.46 μg·L-1 and the average recoveries were 73.2%-119.7% at three levels. CONCLUSION The method is rapid， sensitive and suitable for the determination of 19 carbamate pesticides in Chinese medicinal materials.