Study on Hemolysis of the Cucurbitacin B Galactosylated Liver-Targeting Liposomes in Vitro
DONG Xiao-hui1， LI Le2， SHI Li2， ZHANG Ling1， LU Yi1， DENG Yi-hui1*
Author information+
1. Shenyang Pharmaceutical University， Shenyang 110016，China；2. Liaoning Institute of Pharmaceutical Research， Shenyang 110015，China
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History+
Received
Revised
Published
2011-11-11
2011-11-11
2012-01-01
Issue Date
2011-02-05
Abstract
OBJECTIVE To investigate hemolysis caused by the interaction between rabbit erythrocytes and cucurbitacin B galactosylated liver-targeting liposomes prepared with different adjuvants. METHODS The hemolytic phenomena of the three cucurbitacin B galactosylated liver-targeting liposomes formulations were observed by macroscopic observation and the optical microscope photos of the rabbit erythrocytes were taken with digital microscope. After being treated with a proper emulsion-breaker， the hemolysis percentage of rabbit erythrocytes induced by liposomes were spectrophotometrically determined at 575 nm. RESULTS The three formulations of cucurbitacin B galactosylated liver-targeting liposomes with different adjuvants did not show any hemolytic behavior. The hemolysis percentage of the three formulations were all below 5% by using 10%Triton X-100 as the emulsion-breaker. CONCLUSION Three cucurbitacin B galactosylated liver-targeting liposomes formulations are injectable. The union of macroscopic observation， the optical microscope observation and the spectrophotometry are reliable and feasible to evaluate the hemolytic behavior. 10%Triton X-100 can be used in the hemolysis test as a good emulsion-breaker for liposomes.
DONG Xio-hui;LI Le;SHI Li;ZHNG Ling;LU Yi;DENG Yi-hui.
Study on Hemolysis of the Cucurbitacin B Galactosylated Liver-Targeting Liposomes in Vitro[J]. Chinese Pharmaceutical Journal, 2011, 46(3): 194-198
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