Study on the Intestinal Absorption Kinetics of Schizonepetin in Rats
GENG Ting1, ZHU Hui1, DING An-wei1,2, ZHANG Li1
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1. Nanjing University of Chinese Medicine, Nanjing 210046, China; 2. Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing 210046, China
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Received
Revised
Published
2011-11-11
2011-11-11
2011-12-15
Issue Date
2011-12-15
Abstract
OBJECTIVE To investigate the absorption kinetics of schizonepetin in intestines of rats. METHODS In situ recirculation model was established in rats. The volume of recirculation fluid was adjusted by phenol red. The contents of schizonepetin at different point time were detected by HPLC. RESULTS The absorption rate constant (Ka) of schizonepetin at low, middle and high concentrations (1.84, 3.68 and 7.36 μg·mL-1) were (0.202 8±0.007 0), (0.158 9±0.011 4) and (0.134 2±0.012 2) h-1, respectively, showing significant differences (P<0.05). The Ka of schizonepetin at concentration of 3.68 μg·mL-1 at duodenum, jejunum and ileum were (0.023 8±0.000 6), (0.012 5±0.000 5) and (0.016 5±0.000 8) h-1, respectively (P<0.05). Bile duct ligation and intestinal flora had little influence on the intestinal absorption of schizonepetin. The Ka of P-gp inhibitor group had significant difference with that of the control group (P<0.05). CONCLUSION The absorption of schizonepetin follows first-order process with active diffusion mechanism. Schizonepetin can be absorbed at all intestinal segments, and duodenum is the best absorption site.
GENG Ting;ZHU Hui;DING n-wei;ZHNG Li.
Study on the Intestinal Absorption Kinetics of Schizonepetin in Rats[J]. Chinese Pharmaceutical Journal, 2011, 46(24): 1911-1914
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