WANG Hai-ying, GUO Xue-ping, LUAN Yi-hong, DU Guo-hui
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Shandong Freda Biopharm Co. Ltd., Jinan 250101, China
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History+
Received
Revised
Published
2011-11-11
2011-11-11
2011-12-05
Issue Date
2011-12-05
Abstract
OBJECTIVE To establish a HPLC method for determination of sodium hyaluronate. METHODS Sodium hyaluronate was specifically hydrolyze by hyaluronidase from Streptococcus zooepidemicus in 0.3 mol·L-1 sodium acetate buffer (pH 6.0) at 37 ℃ for 4 h. The mixture was diluted with mobile phase for analysis. The chromatography was performed on a Phenomenex Luna NH2 (4.6 mm× 250 mm, 5 μm) column with 0.4 mol·L-1 sodium dihydrogen phosphate as the mobile phase. The eluent was detected at 232 nm. RESULTS This method had excellent linearity in the range of 0.01-0.1 mg·mL-1 (n=6，r=0.999 8) and good precision. The recovery was 100.4% with RSD of 0.8%. The determination RESULTS were not affected by other ingredients in the samples. CONCLUSION This method is convenient, accurate and specific for determination of sodium hyaluronate.
WNG Hi-ying;GUO Xue-ping;LUN Yi-hong;DU Guo-hui.
Determination of Sodium Hyaluronate by HPLC[J]. Chinese Pharmaceutical Journal, 2011, 46(23): 1844-1846
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References
[1] KUO W J. Practical Aspects of Hyaluronan Based Medical Products[M]. New York： CRC Press, 2005. [2] PE 6. 3 [S]. 2007： 4300-4302. [3] GUO X P. Expression of a novel hyaluronidase from Streptococcus zooepidemicus in E. coli and its application for the preparation of HA oligosaccharides [J]. Carbohydr Polym, 2009, 77： 254-260. [4] WANG H Y. Determination of relative molecular mass and its distribution of sodium hyaluronate by size exclusion chromatography[J]. Food and Durg (食品与药品), 2010, 12(7)： 232-235.
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