Purification and Antitumor Activities of Polysaccharide from Ornithogalum caudatum Ait
CHEN Rui-zhan1， LI Shi-zhe1， LIU Zhi-qiang2*， DONG Hang1， LI Yuan1，YANG Si-min1
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1. College of Chemistry， Changchun Normal University， Changchun 130032， China； 2. Changchun Institute of Applied Chemistry， Chinese Academy of Sciences， Changchun 130022， China
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Received
Revised
Published
2011-11-11
2011-11-11
2011-11-05
Issue Date
2011-11-05
Abstract
OBJECTIVE To investigate the extraction， purification， preliminary characterization and antitumor activities of a water-soluble polysaccharide from Ornithogalum caudatum Ait (OCA). METHODS A homogenization polysaccharide (OCAP-2-2) was obtained by hot water extraction， ethanol precipitation and deproteination with Sevag method， then fractionated by DEAE-Sepharose fast-flow column chromatography， and purified by Sephadex G-75 gel-permeation column chromatography. The sugar concentrations in OCAP-2-2 were characterized by capillary zone electrophoresis (CZE). The homogeneity and molecular weight of OCAP-2-2 were determined by high performance size-exclusion chromatography (HPGPC). The antitumor activity of OCAP-2-2 was evaluated in vivo， by the growth of transplantable S180 sarcoma in mice. The effects of OCAP-2-2 on the proliferation of K562 cells were determined by MTT method in vitro. RESULTS The purified fraction OCAP-2-2 was a homogenization polysaccharide containing 92.3% carbohydrate， 6.21% uronic acid and 3.68% protein， with a molecular weight of 9.84×104. It contained primarily of xylose (Xyl)， glucose (Glc)， mannose (Man) and galactose (Gal) in the molar ratio of 1.26∶2.16∶0.88∶1.00. OCAP-2-2 presented significantly higher antitumor activity against solid tumor Sarcoma 180 in vivo than the blank control at the concentrations from 0.1 μg·mL-1 to 100 μg·mL-1. The inhibition rate in S180 mice was （53.16±4.23）% (P＜0.001) at the concentration of 100 μg·mL-1. OCAP-2-2 could significantly inhibit the proliferation of K562 cells at all tested concentrations. At the concentration of 0.10 μg·mL-1， the inhibition ratio of OCAP-2-2 was the highest with an inhibition ratio beyond （39.83±7.31）% (P＜0.01). CONCLUSION OCAP-2-2 has pronounced anti-tumor activity， and has the potential to be used as a natural antitumor drug.
CHEN Rui-zhn;LI Shi-zhe;LIU Zhi-qing;DONG Hng;LI Yun;YNG Si-min.
Purification and Antitumor Activities of Polysaccharide from Ornithogalum caudatum Ait[J]. Chinese Pharmaceutical Journal, 2011, 46(21): 1630-1634
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References
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