Effect of Alprostadil on Antithrombotic Function in Rabbit Endothelial Cells Injuried by Hypoxia/Reoxygenation

N Ying-nn;GO Hng

Chinese Pharmaceutical Journal ›› 2011, Vol. 46 ›› Issue (2) : 105-107.

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PDF(687 KB)
Chinese Pharmaceutical Journal ›› 2011, Vol. 46 ›› Issue (2) : 105-107.

Effect of Alprostadil on Antithrombotic Function in Rabbit Endothelial Cells Injuried by Hypoxia/Reoxygenation

  • AN Ying-nan，GAO Hang*
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Abstract

OBJECTIVE To study the effect of alprostadil (PGE1 on antithrombotic function in rabbit endothelial cells injuried by hypoxia/reoxygenation(H/R.METHODS H/R injury model was established with the cultured endothelial cells from rabbits in vitro.The endothelial cells were divided into normal group， model group and treatment groups with different concentrations of PGE1(0.05， 0.20， 0.40 μg·mL-1. PGE1 was added to the liquid of H/R and adjusted to a final concentration mentioned above . Malondialdehyde (MDA content， the activity of superoxide dismutase (SOD， and the nitric oxide (NO content in the culture supernatants and the expression of von Willebrand factor (vWF in cells sampled from different group (normal， model and treatment groups were assayed.RESULTS Compared with normal group，MDA content in model group was increased， while the activity of SOD， NO content and the expression of VWF in model group were reduced（P<0.05. Compared with model group， the activity of SOD and NO content in treatment groups with different concentrations of PGE1were increased， while MDA contents in treatment groups were reduced， and the expression of vWF was enhanced（P<0.05. CONCLUSION PGE1 has protective effect on antithrombotic function in rabbit endothelial cells injuried by H/R.

Key words

alprostadil / antithrombotic function / endothelial cells / hypoxia/reoxygenation

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N Ying-nn;GO Hng. Effect of Alprostadil on Antithrombotic Function in Rabbit Endothelial Cells Injuried by Hypoxia/Reoxygenation[J]. Chinese Pharmaceutical Journal, 2011, 46(2): 105-107

References


[1] HAIDER D G，BUCEK D G， GIURGEA A G， et al.The prostaglandin E-1 analog Alprostadil induces VEGF and eNOS expression in endotheloal cells[J]. Am J Physiol Heart Circ Physiol，2005，289（5：2066-2072.
[2] WANG H，ZHANG J S，SHI Y Q. Study on endothelial cell cultured in vitro[J]. Chin J Integr Med Cardio Cerebrovas Dis(中西医结合心脑血管病杂志， 2008， 6 (1 : 107.
[3] ZHANG Y，YAN T H，YUAN L，et al. Protective effects of salidroside on bovine aortic endothelial cells[J]. Pharm Clin Res(药学与临床研究，2009，17(3:195-199.
[4] KOYAMA T， TEMMA K， AKERA T. Reperfusion-induced contracture develops with a decreasing[Ca2+]i in single heart cells [J]. Am J Physiol，1991， 261 (4：1115-1122.
[5] QIU Y， RUI Y C， LI T J， et al.Vascular endothelialgrowth factor-induced hyperpermeability in bovine coronary endothelia cell regulated by hypoxia and the inhibitory efect of salvianolic acid B[J].Chin J Pharmacol Toxicol（中国药理学与毒理学杂志， 2003， 17(1:17-19.
[6] CHEN J，LIU D，CHEN H P，et al.Mechanisms of chloride in anoxia-reoxygenation injury of cultured rat ventricular myocytes[J]. Chin Pharmacol Bull(中国药理学通报，2007，23(6:724-729.
[7] FANG W T， LI H J， ZHOU L S. Protective effects of prostaglandin E1 on human umbilical vein endothelial cell injury induced by hydrogen peroxide[J]. Acta Pharmacol Sin（中国药理学报， 2010，31(4：485-492.
[8] WANG H G，KE Y S，WANG D G，et al. Effects and mechanism of anti-digoxin antiserum on heart function in myocardial anoxia-reoxygenation injury in rats[J].Chin Pharmacol Bull (中国药理学通报，2007，23(5:662-666.
[9] YANG P Y， ALMOFTI M R， LU L， et al.Reduction of atherosclerosis in cholesterol-fed rabbits and decrease of expressions of intracellular adhesion molecule-1 and vascular endothelial growth factor in foam cells by a water-soluble fraction of Polygonum multiflorum[J].J Pharmacol Sci， 2005， 99(3:294-300.
[10] ROSITO G A，DAGOSTINO R B，MASSARO I，et al. Association between obesity and a prothrombotic state：the Framingham Offspring Study[J].Thromb Haemost，2004，91(4：683-689.
[11] CHAMBLESS L E， SHAHAR A R.Association of transient ischemia attack/stroke symptoms assessed by standardized questionnaire and algorithm with cerebral vascular risk factors and carotid artery wall thickness[J].Am J Pidemiol，1996，144(9:857-866.
[12] ZAREI S，FRIEDEN M，RUBI B，et al，Dopamine modulates von Willebrand factor secretion in endothelial cells Via D2-D4 receptors[J]. J Thromb Haemost，2006，4(7:1588-1595.
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