OBJECTIVE To prepare Hepatic stellate cell targeting Vitamin A-curcumin liposome and to investigate its cytotoxicity in vitro. METHODS Reverse phase evaporation was used to prepare VA-curcumin liposome and the entrapment efficiency and the VA binding rate was determined. Entrapment efficiency， VA binging rate and peroxide value were determined to evaluate the stability of liposome at 4 and 25 ℃ for 30 d. MTT assay was used to investigate the cytotoxicity and targeting ability of the liposome in rat hepatic stellate cells HSC-T6 with high expression of retinol binding protein and human esophageal cancer cells A-549 without expression of retinol binding protein. RESULTS The average entrapment efficiency for curcumin was 89.32%， and binding rate for VA was 61.34%. The liposome stored at 4 ℃ was stable. The RESULTS of MTT assay showed that IC₅₀ of curcumin， curcumin liposome， and VA-curcumin liposome in HSC-T6 cell were 31.53， 14.95 and 8.28 μg·mL-1， respectively. Curcumin liposome and VA-curcumin liposome had same cytotoxic effects in A-549 cell. CONCLUSION The optimized conditions were obtained with high entrapment efficiency and good stability. VA-curcumin liposome could specifically target HSC and promote therapeutic effect of curcumin.