OBJECTIVE To study the effects of nitric oxide (NO)， methyl jasmonate (MJ) and salicylic acid (SA) on the phenylethanoid glycoside formation and growth of suspension cultures of Cistanche deserticola. METHODS Suspension cell culture of C. deserticola was established and treatment using sodium nitroprusside (As NO donor)， MJ and SA was carried out. Content determination of echinacoside and total phenylethanoid glycosides (PeGs) was performed using RP-HPLC and UV-spectrophotometer， respectively. RESULTS NO could not enhance the accumulation of PeGs， but low concentration can promote cells growth. MJ and SA greatly enhanced the accumulation of PeGs and restricted the growth of cells. Besides， their optimal elicitation dosage and addition time were different. Maximal PeGs production was achieved with combined treatment using three elicitors at different cell growth stages (0.05 mmol·L-1 sodium nitroprusside added on day 0，50 μmol·L-1 SA on 12nd day and 20 μmol·L-1 MJ added on 16th day). Under this combined treatment， dry weight， total PeGs and echinacoside yield was 1.42，4.17 and 4.02 times of that in untreated cells.CONCLUSION Three elicitors had different effects on the accumulation and growth of suspension cell culture， and the combined treatment of three elicitors resulted in significant increase of the yield of secondary metabolites in C. deserticola suspension cells.
Key words
nitric oxide /
methyl jasmonate /
salicylic acid /
Cistanche deserticola Ma /
suspension cells /
elicitor
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