Partition Coefficients of Hydroxyl and Methoxy Substituted Benzaldehyde Compounds in Sodium Bisulfite-Ethyl Acetate-Water Two-Phase Solvent System
WANG Jin1，CHAO Zhi-mao1*，WANG Xiu-juan2，XI Yan-bin1，GONG Mu-xin2，WANG Di2
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1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China；2. Capital Medical University School of Traditional Chinese Medicine，Beijing 100069，China
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Received
Revised
Published
2011-11-11
2011-11-11
2011-07-05
Issue Date
2011-07-05
Abstract
OBJECTIVE To determine the partition coefficients of 4 hydroxyl and methoxy substituted benzaldehyde compounds in sodium bisulfite-ethyl acetate-water two-phase solvent system，and study the possibility of extracting natural aldehyde compounds by means of sodium bisulfite addition reaction and hydrochloric acid acidulation. METHODS Liposoluble aldehyde compounds were transformed into water soluble sodium α-hydroxyalkane sulfonate derivatives by a sodium bisulfite addition reaction，then transferred into the aqueous phase in hydrophobic two-phase solvent system. The partition coefficients of 4 hydroxyl and methoxy substituted benzaldehyde compounds were measured by high performance liquid chromatography in sodium bisulfite-ethyl acetate-water two-phase solvent system of different ratios. The original aldehyde compounds were recovered from sodium α-hydroxyalkane sulfonate derivatives by adding hydrochloric acid and the second extraction. RESULTS The partition coefficients of aldehyde compounds decreased quickly along with the increasing ratios of sodium bisulfite in sodium bisulfite-ethyl acetate-water two-phase solvent system，and then reached a balance. CONCLUSION Liposoluble aldehyde compounds can be transformed into water soluble sodium α-hydroxyalkane sulfonate derivatives by sodium bisulfite addition reaction and then recovered by hydrochloric acid acidulation. Some natural aldehyde compounds can be specificly extracted and isolated by sodium bisulfite addition reaction followed by acidulation and twice partition extractions.
WNG Jin;CHO Zhi-mo;WNG Xiu-jun;XI Yn-in;GONG Mu-xin;WNG Di.
Partition Coefficients of Hydroxyl and Methoxy Substituted Benzaldehyde Compounds in Sodium Bisulfite-Ethyl Acetate-Water Two-Phase Solvent System[J]. Chinese Pharmaceutical Journal, 2011, 46(13): 1034-1037
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