Preparation and Anti-Tumor Effect of Oridonin Loaded Cholesteryl Formate-Graft Chitosan Copolymer Nano-micelles

ZHO Yong-xing;ZHO Yng;ZHNG Zhen-zhong;ZHNG Xue-xio;SUN Qin

Chinese Pharmaceutical Journal ›› 2011, Vol. 46 ›› Issue (13) : 1015-1019.

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PDF(1348 KB)
Chinese Pharmaceutical Journal ›› 2011, Vol. 46 ›› Issue (13) : 1015-1019.

Preparation and Anti-Tumor Effect of Oridonin Loaded Cholesteryl Formate-Graft Chitosan Copolymer Nano-micelles

  • ZHAO Yong-xing,ZHAO Yang,ZHANG Zhen-zhong,ZHANG Xue-xiao,SUN Qian
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Abstract

OBJECTIVE To prepare oridonin-loaded cholesteryl formate-graft chitosan copolymer (abbreviated as ORI/CF-CS) nano-micelles and study the in vitro anti-tumor activity. METHODS Cholesteryl formate-graft chitosan copolymer (abbreviated as CF-CS) was synthesized via amidation reaction. ORI/CF-CS nano-micelles were prepared by evaporating and characterized by drug loading content,entrapment efficiency,morphology,particle size,and ξ potential. MTT test were applied to assess the cytotoxicity of ORI/CF-CS nano-micelles on Hela cells line. RESULTS The prepared ORI/CF-CS nanomicelles had loading content of 9.16% and entrapment efficiency of 48.83%,small size range of 68.10-113.8 nm,ξ potential range of -34.97--29.19 mV,and slow release in vitro. The cytotoxicity of ORI/CF-CS nanomicelles was stronger than that of oridonin solution. IC50 value of ORI/CF-CS nanomicelles was about 3 times lower,than those of oridonin solution. CONCLUSION CF-CS nano-micelles might be used as potential carriers of oridonin for cancer therapy.

Key words

oridonin / cholesteryl formate-graft chitosan copolymer / nano-micelles / in vitro anti-tumor effect

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ZHO Yong-xing;ZHO Yng;ZHNG Zhen-zhong;ZHNG Xue-xio;SUN Qin. Preparation and Anti-Tumor Effect of Oridonin Loaded Cholesteryl Formate-Graft Chitosan Copolymer Nano-micelles[J]. Chinese Pharmaceutical Journal, 2011, 46(13): 1015-1019

References


[1] LU B. Study on polymersome micelles and niosomes as the vehicle of medicational system[J]. J Henan Univ (Med Ed) (河南大学学报:医学版),2008,27(1):1-7.
[2] ZHAO Y X,HUA H Y,LIANG W Q. Preparation and in vitro anticancer effect of oridonin nanoparticles[J]. Chin Hosp Pharm J (中国医院药学杂志)2008,28(11): 865-867.
[3] ZHANG D R,REN T C. Pharmaceutical development of oridonin [J]. Chin Pharm J (中国药学杂志),2003,38 (11) : 817-820.
[4] BRENNER E,BALDWIN R M,TAMAGNAN G. Asymmetric synthesis of (+)-(S,S)-reboxetine via a new (S)-2-(hydroxymethyl)morpholine preparation[J]. Org Lett,2005,7(5):937-939.
[5] YU J M,LI Y J,OIU L Y,et al. Self-aggregated nanoparticles of cholesterol-modified glycol chitosan conjugate: preparation,characterization and preliminary assessment as a new drug delivery carrier[J]. Eur Polym J,2008,44(3):555-565.
[6] DU Y Z,WANG L,YUAN H,et al. Preparation and characteristics of linoleic acid-grafteded chitosan oligosaccharide micelles as a carrier for doxorubicin[J]. Colloids Surf B Biointerfaces,2009,69(2): 257-263.
[7] WANG Y,QIAO M X,SHEN Q W,et al. Preparation of vinpocetine-loaded amphiphilic chitosan copolymer self-assembled micelles[J]. Chin J New Drugs (中国新药杂志),2007,16(18):1495-1498.
[8] MOSMANN T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays[J]. J Immunol Methods,1983,65(1-2):55-63.
[9] ZHAO Y X,HUA H Y,CHANG M,et al. Preparation and cytotoxic activity of hydroxycamptothecin nanosuspensions[J]. Int J Pharm,2010,392(1-2):64-71.
[10] INOMATA K,SHIMIZU H,NOSE T. Phase equilibrium studies on rod/solvent and rod/coil/solvent systenlS containing poly (a,L-glutamate) having oligo(ethylene glycol)side chains [J]. J Polym Sci Part B: Polym Phys,2000,10(38):1331-1340.
[11] ROLAND I,PIEL G,DELATTRE L,et al. Systematic characterization of oil-in-water emulsions for formulation design[J]. Int J Pharm,2003,263(1/2):85-94.
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