School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001, China
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History+
Received
Revised
Published
2011-11-11
2011-11-11
2011-07-05
Issue Date
2011-07-05
Abstract
OBJECTIVE To prepare oridonin-loaded cholesteryl formate-graft chitosan copolymer (abbreviated as ORI/CF-CS) nano-micelles and study the in vitro anti-tumor activity. METHODS Cholesteryl formate-graft chitosan copolymer (abbreviated as CF-CS) was synthesized via amidation reaction. ORI/CF-CS nano-micelles were prepared by evaporating and characterized by drug loading content,entrapment efficiency,morphology,particle size,and ξ potential. MTT test were applied to assess the cytotoxicity of ORI/CF-CS nano-micelles on Hela cells line. RESULTS The prepared ORI/CF-CS nanomicelles had loading content of 9.16% and entrapment efficiency of 48.83%,small size range of 68.10-113.8 nm,ξ potential range of -34.97--29.19 mV,and slow release in vitro. The cytotoxicity of ORI/CF-CS nanomicelles was stronger than that of oridonin solution. IC50 value of ORI/CF-CS nanomicelles was about 3 times lower,than those of oridonin solution. CONCLUSION CF-CS nano-micelles might be used as potential carriers of oridonin for cancer therapy.
ZHO Yong-xing;ZHO Yng;ZHNG Zhen-zhong;ZHNG Xue-xio;SUN Qin.
Preparation and Anti-Tumor Effect of Oridonin Loaded Cholesteryl Formate-Graft Chitosan Copolymer Nano-micelles[J]. Chinese Pharmaceutical Journal, 2011, 46(13): 1015-1019
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References
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