OBJECTIVE To establish the HPLC fingerprints for assessing the quality of Fructus Aurantii from different habitats，and to determine the contents of naringin， neohesperidin and synephrine. METHODS HPLC fingerprinting and content determination methods were applied to evaluate 10 batches of Fructus Aurantii from different habitats.The samples were separated by Alltima C₁₈ column(4.6 mm×250 mm，5 μm using acetonitrile-0.1％ phosphoric acid and 0.1％ SDS water solution as mobile phase with gradient elution.The flow rate was 1.0 mL·min-1. The detection wavelengths were 224 nm for fingerprinting， 283 nm for content determination of naringin and neohesperidin， and 224 nm for content determination of synephrine， respectively.RESULTS HPLC fingerprint of Fructus Aurantii was established with good separation and repeatability，which could be used for quality assessment of Fructus Aurantii.Six common peaks were defined in characteristic fingerprints， and similarity evaluation system was applied to evaluate the fingerprints of the 10 batches of Fructus Aurantii. The contents of naringin， neohesperidin and synephrine in Fructus Aurantii from different habitats were obviously different from each other. CONCLUSION The method is sensitive， repeatable and accurate， which can be used for the quality control of Fructus Aurantii.The contents of naringin， neohesperidin and synephrine vary significantly in Fructus Aurantii from different habitats， which suggests that more attention should be paid to the clinical use of Fructus Aurantii.