Determination of Saikosaponin b1 and b2 in Chai-Huang Tablet by HPLC
LI Jun, JIANG Hua, ZHANG Yan-ping, WANG Xin-sheng, LI Xiao-ming
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College of Chemical and Pharmaceutical Engineering, Henan University of Science and Technology, Luoyang 471003, China
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History+
Received
Revised
Published
2011-11-11
2011-11-11
2012-01-01
Issue Date
2011-01-05
Abstract
OBJECTIVE To establish a HPLC for the quantitative determination of saikosaponin b1 and b2 in Chai-huang Tablet. METHODS The HPLC method was performed on a Hypersil C18 column (4.6 mm×250 mm, 5 μm with MeOH - Water (75∶25 as the mobile phase. The flow rate was 1.0 mL·min-1. The UV detection wavelength was 254 nm， and the column temperature was set at 30 ℃. RESULTS The calibration curves showed good linear response ranged from 0.015 to 0.120 μg (r=0.999 5 for saikosaponin b1 and from 0.057 to 0.456 μg (r=0.999 8 for saikosaponin b2. The average recoveries were 97.2% (RSD=2.0% and 96.9% (RSD=1.6% respectively. CONCLUSION The method is simple, reliable and repeatable, and can be used for the quantitative determination of saikosaponin b1 and b2 in Chai-huang Tablet.
LI Jun;JING Hu;ZHNG Yn-ping;WNG Xin-sheng;LI Xio-ming.
Determination of Saikosaponin b1 and b2 in Chai-Huang Tablet by HPLC[J]. Chinese Pharmaceutical Journal, 2011, 46(1): 61-63
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References
[1] SUN Y C, JIA Q X. Content determination of Baicaline of Chaihuang Tablets by RP-HPLC [J]. China Pharmacist（中国药师，2003，6（6：355-356. [2] LIU L J，LIU L P，LIU B. Content determination of Baicaline of Chaihuang Tablets by ultraviolet spectrophotometry [J]. Gansu J Tradit Chin Med（甘肃中医，2008，21（1：49-50. [3] Ch. P（2010VolⅠ (中国药典2010年版.一部 [S]. 2010：994. [4] BAI X M，TIAN J M，WANG D B. HPLC Determination of saikosaponin a and d from different areas[J]. Chin Tradit Pat Med（中成药，2006，28（3：440-441.
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