Determination of the Content of Galangin in Alpinia officinarum Hance Harvested in Different Monthes by RP-HPLC

DENG Yi-feng;FENG Li-n;LUO Hui

Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (20) : 1593-1595.

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PDF(713 KB)
Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (20) : 1593-1595.

Determination of the Content of Galangin in Alpinia officinarum Hance Harvested in Different Monthes by RP-HPLC

  • DENG Yi-feng， FENG Li-na，LUO Hui
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Abstract

OBJECTIVE To establish a RP-HPLC method for the determination of the content of galangin in Alpinia officinarum Hance. METHODS An optimum HPLC conditions with binary mobile phase system were obtained. The samples were separated on a Shim-pack VP-ODS column（2.0 mm×250 mm，5 μm）. The mobile phase consisted of 0.1％HAc aqueous solution as phase A and 15％ acetonitrile+40％ methanol+45％ tetrafuran as phase B. Gradient elution program was as followed: 0-20 min， 39%-61%B; 20-30 min， 61%～100%B; 30-40 min， 100%B. The flow rate was 0.2 mL·min-1 ， column temperature was 35 ℃ and UV was at 280 nm. RESULTS The chromatographic resolution of glangin was above 1.5. A good linearity was obtained with the correlation coefficients ranged of 0.020-0.64 g·L-1 (r=0.999 8).The mean recovery was 100.7%，RSD was 2.82%（n=6）. CONCLUSION The method is reliable，accurate and specific， which can be used for the quality control of Alpinia officinarum Hance and its products.

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HPLC / galangin / galangal

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DENG Yi-feng;FENG Li-n;LUO Hui. Determination of the Content of Galangin in Alpinia officinarum Hance Harvested in Different Monthes by RP-HPLC[J]. Chinese Pharmaceutical Journal, 2010, 45(20): 1593-1595

References


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