Influence of Xueshuantong Injection on P450 Activities Using a Cocktail Method

LIU Shi-ji;JU Wen-zheng;CHEN Wei-ko;XU Li-jun;XIONG Ning-ning;TN Heng-shn

Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (2) : 115-118.

PDF(802 KB)
PDF(802 KB)
Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (2) : 115-118.

Influence of Xueshuantong Injection on P450 Activities Using a Cocktail Method

  • LIU Shi-jia1,2,JU Wen-zheng2*,CHEN Wei-kao1,XU Li-jun1 ,XIONG Ning-ning2,TAN Heng-shan3
Author information +
History +

Abstract

OBJECTIVE To evaluate the effects of Xueshuantong Injection on P450 activities by Cocktail probe drugs in rats.METHODS Three probe drugs (theophylline, dapsone, chlorzoxazone) were simultaneously given to rats after middle and high dosing of Xueshuantong injection. The plasma concentrations of the three probes were measured by HPLC and their corresponding pharmacokinetic parameters were calculated. RESULTS There were no differences on theophylline and dapsone plasma concentrations and the corresponding pharmacokinetic parameters in rats between before and after Xueshuantong injection administration for 14 d. But AUC0-t and the concentration of chlorzoxazone were decreased significantly (P< 0.05) and the CL/F was increased in rats after Xueshuantong Injection for 14 consecutive days. No influences were observed on t1/2 and Vd/F.CONCLUSION Xueshuantong injection can induce CYP2E1 activity significantly, but it shows no effect on CYP3A4 and CYP1A2.

Key words

Cocktail / probe / Xueshuantong injection / CYP450

Cite this article

Download Citations
LIU Shi-ji;JU Wen-zheng;CHEN Wei-ko;XU Li-jun;XIONG Ning-ning;TN Heng-shn. Influence of Xueshuantong Injection on P450 Activities Using a Cocktail Method[J]. Chinese Pharmaceutical Journal, 2010, 45(2): 115-118

References


[1] CHEN X Q, JIN Y Y.New Edition Materia Medica(新编药物学)[M]. 15th ed. Beijing: People′s Medical Publishing House, 2003: 732.
[2] FU P, LIU Z Y. Determination of the effecient elements of Xuesai-tong for injection, Xueshuantong injection and Xuemaitong for injection by fingerprint[J]. West China Pharm Sci(华西药学杂志),2007, 22(1): 76-78.
[3] LIU Y,JIAO J J,LOU J S. Progress of“Cocktail”probe substrates approaches[J].Chin J Clin Pharmacol Ther(中国药理学与治疗学杂志),2006,11 (11):1225-1229.
[4] WRIGHTON S A, VANDEN-BRANDEN M, RING B J.The human drug metabolizing cytochromes P450[J]. J Pharmacokinet Biopharm, 1996, 24(5):461-473.
[5] DU Y, ZHU R H, SU F H,et al. Effect of oleanolic acid on CYP1A2, CYP2E1 and CYP3A4 activity in healthy subjects[J]. Chin Pharm J(中国药学杂志), 2008, 43(4):300-303.
[6] XIAO Y J, JIANG Z Z, YAO J C, et al. Investigation of picroside II′s impacts on the P450 activities using a cocktail method[J]. Chin J Nat Med(中国天然药物), 2008, 6 (4):292-297.
[7] YAN S L,HU J P,XU Y X, et al. Simultaneous evaluation of activity of hepatic CYP450 following administration of Fisch Fructus Forsythiae Cocktail probe drugs[J]. Chin Pharm J(中国药学杂志),2003, 38(10):761-763.
[8] HU J P, YAN S L, TANG J C, et al. Simultaneous evaluation of effect of rhizoma Curcumae Longae on hepatic CYP450 by cocktail probe drugs[J]. J Cap Univ Med Sci(首都医科大学学报), 2004, 25(4):427-430.
[9] SHI J, CHEN A J, ZHANG F, et al. Effects of traditional Chinese medicine Panax notoginoside and Tetramethylpyrazine on cytochrome P450 subtype enzymes[J]. Chin J Integr Tradit West Med Intens Crit Care (中国中西医结合急救杂志), 2008, 15(6):342-345.
PDF(802 KB)

Accesses

Citation

Detail

Sections
Recommended

/