Determination of Oleanolic Acid and Ursolic Acid in the Different Parts of Lavandula angustifolia Mill. by HPLC-PAD
ZOU Sheng-qin
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Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines， College of Chemistry and Bioengineering， Yichun University， Yichun 336000，China
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History+
Received
Revised
Published
2012-01-01
2012-01-01
2012-01-01
Issue Date
2010-06-15
Abstract
OBJECTIVE To determine oleanolic acid and ursolic acid in Lavandula angustifolia Mill.. METHODS The separation was performed on a Kromasil C18 column (4.6 mm×250 mm，5 μm). The mobile phase consisted of methanol-water-phosphoric acid (90∶10∶0.1). The flow rate was 0.8 mL·min-1 at 25 ℃，and the detection wavelength was set at 210 nm. RESULTS The linear ranges of oleanolic acid and ursolic acid were 0.50 to 9.00 μg and 0.92 to 16.56 μg， respectively. The average recoveries (n=9) for oleanolic acid and ursolic acid were 98.1% (RSD=1.8%) and 98.3%(RSD=1.2%)， respectively. CONCLUSION This method is accurate， reliable and sensitive for the determination of oleanolic acid and ursolic acid in Lavandula angustifolia.
ZOU Sheng-qin.
Determination of Oleanolic Acid and Ursolic Acid in the Different Parts of Lavandula angustifolia Mill. by HPLC-PAD[J]. Chinese Pharmaceutical Journal, 2010, 45(12): 949-951
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References
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