Application of Monolithic-column on HPLC Specific Chromatograms of Traditional Chinese Medicines

XIONG Ye-jun;FN Song-hu

Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (12) : 940-944.

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PDF(1738 KB)
Chinese Pharmaceutical Journal ›› 2010, Vol. 45 ›› Issue (12) : 940-944.

Application of Monolithic-column on HPLC Specific Chromatograms of Traditional Chinese Medicines

  • XIONG Ye-juan1，FAN Song-hua2
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Abstract

OBJECTIVE To explore the application of monolithic-column to specific chromatograms analysis of traditional Chinese medicine (TCM). METHODS The fingerprint determined by HPLC-UV HPLC system equipbed with the monolithic-column was compared with the fingerprint from several conventional particulate columns by various gradient-elute methods， respectively. Radix. glycyrrhiza， Salvia miltiorrhiza as well as Radix et rhizoma rhei. were selected as samples. RESULTS The monolithic-column system obtained equivalent or higher resolution than particulate columns， while consumed 50% or less time. CONCLUSION It can be concluded monolithic-column is helpful for the specific chromatograms analysis and quality control of TCM， especially in the analysis efficiency， the development and conversion of the analysis method.

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monolithic-column / HPLC / specific chromatograms

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XIONG Ye-jun;FN Song-hu. Application of Monolithic-column on HPLC Specific Chromatograms of Traditional Chinese Medicines[J]. Chinese Pharmaceutical Journal, 2010, 45(12): 940-944

References


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