Protection of Intranasal Delivery of Calcitonin Gene-related Peptide Against Cerebral Injury Following Subarachnoid Hemorrhage
SUN Bao-liang1， CHI Shu-mei1，2， YANG Ming-feng1， ZHANG Yan-bo1， YUAN Hui1
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1. Key Lab of Cerebral Microcirculation， College and University of Shandong Province， Department of Neurology ， Affiliated Hospital of Taishan Medical College， Tai′an 271000， China; 2.Department of Neurology， Central Hospital of Binzhou， Binzhou 251700， China
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History+
Received
Revised
Published
2012-01-01
2012-01-01
2012-01-01
Issue Date
2010-06-15
Abstract
OBJECTIVE To investigate the protective effects of intranasal delivery of calcitonin gene-related peptide (CGRP) on cerebral injury following subarachnoid hemorrhage (SAH). METHODS Wistar rats were divided into normal control group， SAH group， intranasal NS+SAH group and intranasal CGRP+SAH group. SAH models were produced by double injection of autologous arterial blood into cisterna magna. CGRP and NS were given by intranasal perfusion. On the third day after second cisternal injection， the apoptosis of cells in cerebral cortex was determined by a TUNEL staining. The expression of Bcl-2 protein in cerebral cortex was observed by immunofluorescence method combined with laser confocal microscopic observation. RESULTS Anatomic observation revealed SAH models were successfully manufactured. A great deal of TUNEL positive cells appeared in cerebral cortex in SAH group and intranasal NS+SAH group. The numbers of TUNEL positive cells in cerebral cortex in intranasal+SAH CGRP+SAH group decreased， compared with those in intranasal NS+SAH group. Increased expressions of Bcl-2 protein in cerebral cortex in SAH and intranasal NS+SAH group were found， compared with normal control group. The expression of Bcl-2 in intranasal CGRP group was more obvious than that in intranasal NS+SAH group. CONCLUSION Intranasal delivery of CGRP may alleviate cerebral injury following SAH.
SUN o-ling;CHI Shu-mei;YNG Ming-feng;ZHNG Yn-o;YUN Hui.
Protection of Intranasal Delivery of Calcitonin Gene-related Peptide Against Cerebral Injury Following Subarachnoid Hemorrhage[J]. Chinese Pharmaceutical Journal, 2010, 45(12): 909-912
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References
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