OBJECTIVE To study the effect of meropenem
<> on the biofilms of
<> P. aeruginosa <>las/rhl mutation
<> in vitro . METHODS The MIC and MBC of meropenem (MEM) were measured in
<>P. aeruginosa PAO1,
lasI rhlI and
lasR rhlR mutants. The biofilms of PAO1,
lasI rhlI and
lasR rhlR mutants were incubated in a po lyvinyl chloride tube in 0.9% saline for three days ， and then immersed in meropenem solution of 26 mg·L
-1 for 24 hours ， followed by examinations of scanning electron i c microscopy (SEM). RESULTS (1) The MIC and MBC of MEM in PAO1,
lasI rhlI and
lasR rhlR mutants were 1 and 2 mg·L
-1 respectively. （ 2 ） After 3 day’s incubation, PAO1 biofilms showed a well-developed structure. However, the biofilms of
lasR rhlR and
<>lasI rhlI mutants were thin and poor developed. After incubation for 24 hours in meropenem solution, PAO1 biofilm became pellicle-shaped ， whereas
<>lasR rhlR and
<>lasI rhlI mutants biofilms were almost destructed and there were only small pieces left. CONCLUSION
<>las/rhl genes play probably an important role in the formation of
<>P.aeruginosa biofilm and the resistance to meropenem.
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Key words
biofilms /
quorum sensing /
Pseudomonas aeruginosa /
meropenem
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References
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（ 收稿日期 ： 2008-12-22 ）
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