OBJECTIVE To establish a RP-HPLC method for the determination of albendazole (ABZ), albendazole sulphoxide (ABZSO) and albendazole sulphone(ABZSO2) in human plasma. METHODS The drugs was extracted from plasma with C2 column by SPE method. The compounds were separated on an Agilent Zorbax RX-C8 column（4.6 mm×250 mm，5 μm）by Waters 2690 HPLC. The mobile phase consisted of acetonitrile-NaAc buffer (0.1 mol·L-1, adjusted to pH 5.0 with acetic acid). The detection wavelength was 291 nm. The flow rate was 1.0 mL·min-1. RESULTS A good linearity was obtained over the range of 10~600 μg·L-1 for ABZ, 10~1 000 μg·L-1 for ABZSO and 10~300 μg·L-1 for ABZSO2. The recoveries of method were from 92.23%~103.81% for ABZ、99.59%~100.21% for ABZSO, 96.89%~106.84% for ABZSO2. The precisions of within-run were 2.03%~3.12%, 2.05%~3.80% and 2.79%~4.14%, respectively, and the precisions of between-run were 3.20%~4.33%, 2.05%~3.48% and 2.87%~3.87%, respectively. CONCLUSION The method is sensitive, accurate and reliable, and can be used in clinical research of ABZ.
Key words
albendazole /
albendazole sulphoxide /
albendazole sulphone /
plasma drug level
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References
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（ 收稿日期 ： 2009-03-17 ）
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