OBJECTIVE To investigate the protective effects and mechanisms of Dengzhan Xixin injection on cardiac microvascular endothelial cells (CMECs) induced by TNF-α. METHODS After incubating CMECs with TNF-α (500 u×mL-1), cell vitality and cellular impairment were determined by MTT assay and lactate dehydrogenase (LDH) release. IL-6 and MCP-1 secretion were examined by ELISA. RT-PCR was employed to assess the mRNA expression of IL-6 and MCP-1. RESULTS Dengzhan Xixin injection groups (12.5, 25, 50 and 100 mg×L-1) enhanced the vitality of CMECs and significantly inhibited the release of LDH. Dengzhan Xixin injection groups (50 and 100 mg×L-1) reduced the secretion and mRNA expression of IL-6 and MCP-1 after 6 h incubation of TNF-α with CMEC. CONCLUSION Dengzhan Xixin injection could inhibit TNF-α-stimulated IL-6 and MCP-1 release and down-regulate mRNA expression of IL-6 and MCP-1 in CMECs. Dengzhan Xixin injection can attenuate damage of endothelial cells and may play an important role in suppressing flammation reaction induced TNF-α in CMECs.
Key words
Dengzhan Xixin injection /
rat cardiac microvascular endothelial cells /
tumor necrosis factor /
interleukin-6 /
monocyte chemotactic protein-1
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（ 收稿日期 ： 2009-01-02 ）
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