OBJECTIVE To establish a RP-HPLC method for the determination of the content and entrapment efficiency of silybin nano-structured lipid carrier (NLC). METHODS The content of silibin NLC was determined by HPLC.The analytical column was Kromasil C18（4.6 mm×150 mm,5 μm）and the column temperature was ambient．The mobile phase was methanol-0.35 mol·L-1(48∶52)．The flow rate was l mL·min-1．The detection wavelength was 288 nm．Centrifugation ultrafiltration and sephadex gel filtration methods were employed to isolate free silybin for the entrapment efficiency. RESULTS Silybin was linear in the range of 1~40 mg·L-1 (r=0.999 8)，the average recovery was 99.57%(RSD=0.52%)．The entrapment efficiencies of silybin NLC determinated by sephadex gel filtration and centrifugation ultrafiltration were 84.20% and 96.87%, respectively. CONCLUSION ■Centrifugation ultrafiltration combined with HPLC is able to determine the entrapment efficiency of silybin NLC efficiently, accurately and conveniently.
Key words
silybin /
nano-structured lipid carrier /
HPLC /
centrifugation ultrafiltration /
sephadex gel filtration /
entrapment efficiency
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（ 收稿日期 ： 2009-01-11 ）
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