OBJECTIVE To quantitatively analyze anthraquinones in Radix Polygoni Multiflori Praeparata by liquid-phase microextraction (LPME) coupled with high performance liquid chromatography. METHODS With a self-made LPME system, the optimal extraction conditions were as follows: polyvinylidene difluoride hollow fiber as solvent carrier, 1-octanol as extraction solvent, HCl concentration in the donor was 2 mmol·L-1, methanol content in the donor was 50%, the stirring rate was 1 800 r·min-1 and the extraction time was 60 min. After microextraction, the extract was dried in 80 ℃ water bath, dissolved with 60 μL methanol and then analyzed by HPLC at 434 nm. RESULTS Under the optimal conditions, detection limits of aloe-emodin, crysophanol rhein, physcion and emodin were 0.30, 0.25, 0.25, 0.30 and 0.35 μg·L-1, respectively. The average relative standard deviation (precision) ranged from 8.1% to 14.1%. The relative recoveries in Radix Polygoni Multiflori Praeparata ranged from 75.4% to 111.5%. The LPME enrichment factors of the five analytes were 21, 21, 36, 44 and 47 respectively. Emodin, physcion, trace rhein and crysophanol in Radix Polygoni Multiflori Praeparata were determined. CONCLUSION Liquid-phase microextraction is a pre-processing technique which is simple, quick, with high selectivity, high enrichment factor and little organic solvent consumption. It could be applied to simultaneous analysis of anthraquinones in Radix Polygoni Multiflori Praeparata.
Key words
liquid-phase microextraction /
enrichment factor /
anthraquinone /
Radix Polygoni Multiflori Praeparata
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] PEDERSON-BJERGARD S, RASMUSSEN K E. Liquid-liquid- liquid microextraction for sample preparation of biological fluids prior to capilla[J]. <>Anal Chem, 1999, 71: 2650-2656.
[2] BAI X H, YANG X, CHEN X, <>et al. Determination of oxymatrine and matrine by liquid phase microextraction with back extraction coupled with high performance liquid chromatography[J]. <>Chin J Anal Chem ( 分析化学 ), 2008, 36(2): 182-186.
[3] WANG X Y, CHEN X, BAI X H, <>et al. Determination of magnolol and honoliol in traditional Chinese medicine <>Magnolis officinalis and its preparations by liquid-phase microextraction-back extraction combined with high performance liquid chromatography[J]. <>J Chin Pharm Sci ( 中国药学英文版 ), 2008, 17(2):163-166.
[4] WANG X Y, CHEN X, BAI X H, <>et al. The application and extraction mechanism of liquid-liquid-liquid phase microextraction in the analysis of phenylpropionic acids[J]. <>Chin J Anal Chem( 分析化学 ), 2009, 37 (1): 35-40.
[5] LI J P. Recent developments in investigation of pharmacologic actions of Radix Polygoni Multiflori Praeparata[J]. <>China Pharm ( 中国药业 ), 2003, 12(10): 74-75.
[6] LIU Z L, SONG Z Q, ZHANG L, <>et al. Influence of process methods on contents of chemical component Radix Polygoni Multiflori Praeparata[J]. <>China J Chin Mater Med( 中国中药杂志 ), 2005, 30(5):336-340.
[7] WANG W J, ZHANG W M, DONG X L, <>et al. Studies on chemical constituents of Polygonum Multiflori Praepatata from Yunnan[J]. <>Yunnan J Coll Tradit Chin Med( 云南中医学院学报 ), 2005, 28(1): 10-12.
[8] YUAN H L, ZHANG C, LI X Y. Determination of rhein, emodin and physcion in tuber fleeceflower by supercritical fluid extraction-HPLC [J].<> Chin Tradit Herb Med( 中草药 ), 1999, 30(4): 258-260.
[9] WU Z, ZHANG M, ZHANG C F, <>et al. Study on quality evaluation for 36 samples of Radix Polygonui Multiflori Praepatata by HPLC-Fingerprints [J]. <>Chin Pharm J ( 中国药学杂志 ), 2006 , 41(14): 257-260.
[10] LIN R M, LI L, CHEN H S, <>et al. HPLC Determination of 5 anthraquinones in Rhubarb of different breeds from different places of production[J].<> J Chin Med Mater( 中药材 ), 2005, 28(3):197-198 ．
（ 收稿日期 ： 2008-12-16 ）
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
PDF(2895 KB)
