OBJECTIVE To study the expression of myosin light chain kinase(MLCK) in endothelial cell regulated by the melatonin through the pathway of ERK/MAPK in atherosclerosis(AS) model rabbit. METHODS The model rabbit of AS was established and the dynamic variability of the lipids in the serum of model rabbits was analyzed. The MLCK activity of artery tissue of model rabbits was measured by the method of γ-32P-ATP incorporated.The expression of MLCK in the endothelial cell was detected by immunohistochemistry and western blot, meanwhile, the phosphorylation of ERK in the endothelial cell was also detected by western blot. The change of morphology of artery wall in model rabbits was examined according to the HE stain. RESULTS The AS rabbit model was established successfully. After being fed with the high cholesterol (Cho) for 4,8,12 weeks, compared with the normal control, the related biochemistry quotas was increased obviously in the serum (P<0.05),the MLCK activity of artery tissue of model rabbits was increased gradually(P<0.05),the expression of MLCK and the phosphorylation of ERK in the artery tissue were raised gradually. At the same time，the HE staining indicated the increase of foam cells products. When melatonin（MLT） was added into the high Cho food,the activity of MLCK,the permeability of arterial wall,the expression of MLCK and the phosphorylation of ERK in artery tissue were decreased. The aortic tunica intima symptom seem to be reliefed. CONCLUSION MLT may relief the pathological changes of plaque in the AS model rabbit through the pathway of ERK/MAPK to down regulate the activity of MLCK in endothelial cell.
Key words
melatonin /
atherosclerosis /
artery /
myosin light chain kinase
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（ 收稿日期 ： 2008-09-10 ）
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