Determination of Paraquat in Whole Blood by LC/MS/MS Assay
QIU Kui, WU Hua, ZHANG Zheng, XU Jia-jia, YANG Mei, KONG Fan-cui, WANG He-yao*
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(<>Department of Pharmacology, <>Beijing Chaoyang Hospital Affiliate of Capital Medical University Sciences, Beijing 100020 ， <> China
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History+
Received
Revised
Published
2000-01-01
2000-01-01
2009-08-25
Issue Date
2009-08-25
Abstract
OBJECTIVE To develop a LC/MS/MS method for the determination of paraquat assay in whole blood. METHODS The sample were pretreated with the precipitation of protein with acetonitrile and separated on a Waters Symmetry C18 column (4.6 mm×50 mm，5 μm ) with the mobile phase consisting of acetonitrile: water with 0.3% trifluoroacetic acid (95∶5). The flow rate was 0.3 mL·min-1. A API3200 triple quadmass spectrometer system equipped with an electrospray ionization source was used as the detector and operated in the positive ion mode. Selected reaction monitoring (SRM) was used with the precursor to product ion combinations of m/z 185.2→169.1. The method was used to measure paraquat in whole blood of patient. RESULTS ■The average recoveries of paraquat were 71.80%, 70.77% and 77.84% at three concentration levels of 5, 1.25, 0.08 mg·L-1, respectively. The relative standard deviations from intra-day (n=5) and inter-day (n=3) precision were less than 15%. The calibration curves for paraquat had good linearity（r=0.998 8，n=8）over the range of 0.04-10 mg·L-1. The limits of quantification for paraquat was 0.04 mg·L-1. CONCLUSION The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of paraquat blood sample.
QIU Kui;WU Hu;ZHNG Zheng;XU Ji-ji;YNG Mei;KONG Fn-cui;WNG He-yo .
Determination of Paraquat in Whole Blood by LC/MS/MS Assay[J]. Chinese Pharmaceutical Journal, 2009, 44(16): 1259-1261
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References
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