OBJECTIVE To prepare the self-assembled neurotoxin-loaded nanoparticles of core-shell type, and investigate its physicochemical properties and release behavior in vitro. METHODS The self-assembled neurotoxin-loaded nanoparticles of core-shell type were prepared with PEG-g-PECA by emulsion polymerization method. The orthogonal design was used to optimize the preparation technology. The transmission electron microscope(TEM) and Zeta sizer instrument were utilized to investigate the physicochemical properties of the nanoparticles, and the drug release behavior in PBS buffer at pH 7.4 and 6.8 in vitro were studied by dialysis method respectively. RESULTS The mean diameter of the nanoparticles were (89.6±8.9)nm with polydispersity index of (0.110±0.003). The entrapment efficiency was (58.43±0.62)%, and Zeta potential was (-38.81±0.47) mV. The in vitro release profiles of the nanoparticles in pH 7.4 and 6.8 PBS buffer were consistent with Weibull equation, lnln[1/(1-Q)]=0.474lnt-1.612 1,r=0.994 6 (pH 7.4) and lnln[1/(1-Q)]=0.351lnt–0.827 1, r=0.970 8 (pH 6.8) respectively. CONCLUSION PEG-g-PECA is suitable for preparation of self-assembled neurotoxin-loaded nanoparticles of core-shell type with high entrapment efficiency, stable properties and excellent drug sustained release behavior.
Key words
neurotoxin /
self-assembled nanoparticles of core-shell type /
poly(ethylene glycol)-g-polyethylcyanoacrylate block copolymer /
preparation /
in vitro release
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