Antiviral Effect of Adefovir Dipivoxil Loaded Solid Lipid Nanoparticles in Vitro

MIO Jing;ZHNG Xing-guo;JING Si-ping;LI Min-wei;HU Fu-qing;DU Yong-zhong

Chinese Pharmaceutical Journal ›› 2009, Vol. 44 ›› Issue (11) : 853-856.

Chinese Pharmaceutical Journal ›› 2009, Vol. 44 ›› Issue (11) : 853-856.
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Antiviral Effect of Adefovir Dipivoxil Loaded Solid Lipid Nanoparticles in Vitro

  • MIAO Jing1,ZHANG Xing-guo1*,JIANG Sai-ping1,LI Min-wei2,HU Fu-qiang3,DU Yong-zhong3
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Abstract

OBJECTIVE To study antiviral effect of adefovir dipivoxil(ADV) delivered by solid lipid nanoparticles(SLN) in vitro.METHODS ADV loaded SLN(ADV-SLN) were prepared by solvent diffusion method in aqueous system,and the drug entrapment efficiency(EE) and drug loading(DL) were determined by ultraviolet spectrophotometric method at the wavelength of 260 nm.The cellular uptakes of SLN by HepG2.2.15 were investigated by ODA-FITC incorporated SLN and fluorescence microscopy.HBsAg and HBeAg were determined by commercial enzyme immunoassay kits,while HBV DNA was quantified by a commercial real-time polymerase chain reaction(PCR) kit.RESULTS EE and DL were(16.72±2.81)% and(3.86±0.42)% for ADV loaded SLN.HepG2.2.15 increased uptake of SLN effectively and the cellular uptake of SLN was time-dependent.Comparing with free ADV,the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen(HBsAg),hepatitis Be antigen(HBeAg) and hepatitis B virus(HBV) DNA levels in vitro were significantly enhanced.CONCLUSION ADV delivered by NLC could significantly enhance in vitro antiviral effect,which revealed a potential application for the targeting antiviral therapy.

Key words

adefovir dipivoxil(ADV) / solid lipid nanoparticles(SLN) / octadecylamine-fluorescein isothiocynate(ODA-FITC) / hepatitis B virus(HBV)

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MIO Jing;ZHNG Xing-guo;JING Si-ping;LI Min-wei;HU Fu-qing;DU Yong-zhong. Antiviral Effect of Adefovir Dipivoxil Loaded Solid Lipid Nanoparticles in Vitro[J]. Chinese Pharmaceutical Journal, 2009, 44(11): 853-856

References

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