1. Department of Pharmacy,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China;2. State Key Lab for Diagnosis and Treatment of Infectious Diseases,Zhejiang University,Hangzhou 310003,China;3. College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China
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History+
Received
Revised
Published
2008-11-05
2008-11-05
2008-11-05
Issue Date
2009-06-05
Abstract
OBJECTIVE To study antiviral effect of adefovir dipivoxil(ADV) delivered by solid lipid nanoparticles(SLN) in vitro.METHODS ADV loaded SLN(ADV-SLN) were prepared by solvent diffusion method in aqueous system,and the drug entrapment efficiency(EE) and drug loading(DL) were determined by ultraviolet spectrophotometric method at the wavelength of 260 nm.The cellular uptakes of SLN by HepG2.2.15 were investigated by ODA-FITC incorporated SLN and fluorescence microscopy.HBsAg and HBeAg were determined by commercial enzyme immunoassay kits,while HBV DNA was quantified by a commercial real-time polymerase chain reaction(PCR) kit.RESULTS EE and DL were(16.72±2.81)% and(3.86±0.42)% for ADV loaded SLN.HepG2.2.15 increased uptake of SLN effectively and the cellular uptake of SLN was time-dependent.Comparing with free ADV,the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen(HBsAg),hepatitis Be antigen(HBeAg) and hepatitis B virus(HBV) DNA levels in vitro were significantly enhanced.CONCLUSION ADV delivered by NLC could significantly enhance in vitro antiviral effect,which revealed a potential application for the targeting antiviral therapy.
MIO Jing;ZHNG Xing-guo;JING Si-ping;LI Min-wei;HU Fu-qing;DU Yong-zhong.
Antiviral Effect of Adefovir Dipivoxil Loaded Solid Lipid Nanoparticles in Vitro[J]. Chinese Pharmaceutical Journal, 2009, 44(11): 853-856
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References
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