OBJECTIVE To establish a HPLC quantitative method for the determination of imipenem and cilastatin sodium for Injection.METHODS The separation was performed on a CN column(4.6 mm×250 mm,5μm) with mobile phase of acetonitrile -0.1%phosphoric acid(50:50) at the flow rate of 1 mL·min^-1.The determination wavelength was at 265 nm.The column temperature was at 30℃.RESULTS The theoretical plate number of imipenem was 9 000,and that of cilastin sodium was 9 500.The resolution was more than 1.5.The regression equation for imipenem was Y=0.638 6X+6.173(r=0.999 9),and the linear range was 117.1-585.5 mg·L^-1.The regression equation of cilastin sodium was Y=0.588 6X+6.294(r=0.999 9),and the linear range was 117.1-585.3 mg·L^-1.The average recoveries of imipenem and cilastin sodium were 98.8%(1.7%) and 99.2%(1.1%), respectively.CONCLUSION The method is precise,accurate,simple,and can be applied to determin the content of Imipenem and Cilastin Sodium for Injection.