OBJECTIVE To research the extraction and separation of flavonoid in three Chinese materia medics Elshola blanda,Ginkgo biloba Linn and Lysimachia christinae Hance by ultrasonic extraction technology coupling with propyl-alcohol ammonium sulfateaqueous aqueous two-phase separation and determine the antioxidantion activities of extractives.METHODS The contents of general flavonoids were determined by Al(NO3)3-NaNO2 spectrophotometric methods,and the inhibition rate of lipid peroxidation(LPO) induced by·OH free radical generation system was used to evaluate the antioxidantion activities of extractives. RESULTS The extraction rates of general flavonoids were 2.7%-6.12%for three kinds of Chinese materia medics in the optimum extracting conditions,and the general flavonoids contents in extractives were 17.3%-21.1%,much higher than that of reflux extraction of solvent,and the maxium LPO inhibit rates of the extractives of three kinds of Chinese materia medics were between 70.1%-74.4%.CONCLUSION The proposed method could extracte general flavonoids from Chinese materia medics effectively. The extractiuon conditions were mild and friendly environmentally.The extractives could singificantly inhibit LPO induced by·OH free radical.
Key words
ultrasonic extraction /
aqueous two-phase separation /
Chinese materia medic /
flavonoid
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