Abstract
OBJECTIVE To analyze the physico-chemical properties of a heteropolysaccharide extracted from the dry roots of Astragalus membranaceus(Fisch.) Bunge var.mongholious(Bunge) Hsiao.METHODS Astraglus polysaccharide(APS) was obtained using Microwave-assistant extracting technique.Crude APS was deproteinized by the enzymolysis of neutral proteases.The relative molecular mass,chemical components and molecular molar ratio,and main configuration of the purifired APS were obtained by high performance gel filtration chromatography,MP-derivation high performance liquid chromatography,infrared spectra and/or 1H-NMR.Thermoanalysis was carried out with derivative thermogravimetry(DTG) and differential scanning calorimetry(DSC).RESULTS The relative molecular mass of the APS was 1.1×104 with the purity of 97.16%.The heteropoly saccharide consisted of Rha,Glc,Gal and Ara with the molecular molar ratio of 1.19:72.01:5.85:20.95 and its main configuration was α-pyran ring with the principal chain linked by Glc 1→6 glucosidic bond.It was unstable to strong light,heat and humidity.Its effects on immune modulation and antioxidation were evaluated before.CONCLUSION The APS obtained in the experiment is a new heteropolysaccharide from Radix Astragalus.
Key words
astragalus heteropolysaccharide /
chemical components /
structure /
stability
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LI Hong-qun;ZHO Wn-guo;Lü Xio-hu;LI Xio-xu.
Physical and Chemical Analysis of a New Heteropolysaccharide from Radix Astragalus [J]. Chinese Pharmaceutical Journal, 2009, 44(09): 654-657
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References
[1] SHAO B M，XU W，DAI H，et al. A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus，a Chinese medicinal herb[J]. Biochemi Biophys Res Commun，2004，320(4)：1103-1011.
[2] LEE K Y，JEON Y J. Macrophage activation by polysaccharides isolated from Astraglus membranaceus[J]. Int Immunopharmacol，2005，5(7-8)：1225-1233.
[3] MA X Q，SHI Q，DUAN J A，et al. Chemical analysis of Radix Astragali (Huangqi) in China：a comparison with its adulterants and seasonal variations[J]. Agric Food Chem，2002，50(17)： 4861-4866.
[4] LI H Q，ZHAO M C. Optimizing microwave-assisted extraction technology of astragalus polysaccharides with Response surface methodology[J]. J Tradit Chin Vet Med（中国兽医药杂志），2007，26（2）：10-14.
[5] LI H Q，LLOYD R J，WANG J D. Effect of Astragalus polysaccharides on erythrocyte immune adherence of chickens inoculated with infectious bursal disease virus[J]. Agric Sci China（中国农业科学），2007，6(11)：101-105.
[6] LI H Q，WU C H，GAO H，et al. Effect of astragalus polysaccharides on immune function of erythrocytes of chickens infected with IBDV[J]. Chin J Vet Sci（中国兽医科学），2006，36(1)：74-79.
[7] LI H Q，ZHAO M C. Optimizing microwave-assisted extraction technology of astragalus polysaccharides with Response surface methodology[J]. J Tradit Chin Vet Med（中国兽医药杂志），2007，26（2）：10-14.
[8] WANG J S，LIU G L，ZHANG Y，et al. Research Techniques in Agricultural Biochemistry[M]. Beijing：Chinese Agricultural Publishing House，2001：183-184.
[9] YAN Q J，HAN L J，JIANG Z Q. Removal of protein from the crude astragalus polysaccharides by proteases[J]. Food Sci Technol，2004，6：23-26.
[10] WANG M T，XIE P S，WANG Z D. Extraction of Natural Organic Compounds Isolation and Identification[M].Beijing：Chemical Industry Press，2004：86-93，157-162，206-220，418-421.
[11] FANG J N，WEI Y A. Application application of high performance liquid chromatogram in biomedicine[J]. Chin J Chromatogr（色谱），1991，9(2)：103-107.
[12] XU J，ZHANG Q H，ZHANG W B，et al. Applications of fluorescent derivatization in liquid chromatography for carbohydrate analysis[J]. Chin J Chromatogr（色谱），2003，21(2)：115-120.
[13] YANG X B，ZHAO Y，ZHOU S Y，et al. Analysis of Monosaccharide Composition in Angelica Polysaccharides by Precolumn Derivatization High Performance Liquid Chromatography[J]. Chin J Anal Chem，2005，33(9)：1287-1290.
[14] KURATA S，YAMADA K，TAKATSU K，et al. Extracellular acidic polysaccharide production by a two-membered bacterial coculture[J]. Biosci Biotechnol Biochem，2003，67(1)：8-14．
[15] State food and drug administration. Instruction on stability research techniques of Chinese materia medica and naturally occurring drugs.Doc.No.[2006]678.
[16] SHE Z G，HU G P，GUO Z Y，et al. Study on the thermal decomposition of the sulphated polysaccharide hal-A from haliotis diverisicolor reeve[J]. Chin J Org Chem（有机化学），2003，23(10)：1149-1151.
[17] PRETSCH E，BUHLMANN P，AFFOLTER C. Structure Determination of Organic Compounds Tables of Spectral Data[M]. Springer-Verlan GmbH＆Co.KG，2000：245-312.
[18] DEMLEITNER S，KRAUS J，FRANZ G. Synthesis and antitumour activity of sulfoalkyl derivatives of curdlan and lichenan[J]. Carbohydr Res，1992，226(2)：247-252.
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