Optimization of Ethanol Extraction Process and Condition For Xiongqiong Decoction by Orthogonal Test Design and HPLC

RONG Rong;CHEN Ming-qing;Lü Qing-to;SUN Xiu-mei;YNG Yong

Chinese Pharmaceutical Journal ›› 2009, Vol. 44 ›› Issue (08) : 580-584.

Chinese Pharmaceutical Journal ›› 2009, Vol. 44 ›› Issue (08) : 580-584.
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Optimization of Ethanol Extraction Process and Condition For Xiongqiong Decoction by Orthogonal Test Design and HPLC

  • RONG Ronga，CHEN Ming-qianga，Lü Qing-taoa，SUN Xiu-meia，YANG Yongb
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Abstract

OBJECTIVE To optimize the ethanol extraction process and condition of Xiongqiong decoction.METHODS Using high performance liquid chromatography(HPLC) fingerprint technology,the effects of ethanol concentration,ethanol amount,extraction time and the number of extraction times on the yield of extract were evaluated by orthogonal test design with multi-index of the content of ferulic acid,the total area of characteristic peaks of HPLC and the yield of powdered extract.RESULTS The optimum condition of the ethanol extraction was obtained.The raw material amount was extracted with 12 volumes of 70% ethanol three times for 1.5 h per extraction process.CONCLUSION The optimized extraction method was more effective than the traditional extraction method and can be used for the extraction process of Xiongqiong decoction.

Key words

Xiongqiong decoction / ethanol extraction / ferulic acid / HPLC fingerprint / orthogonal test design

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RONG Rong;CHEN Ming-qing;Lü Qing-to;SUN Xiu-mei;YNG Yong. Optimization of Ethanol Extraction Process and Condition For Xiongqiong Decoction by Orthogonal Test Design and HPLC [J]. Chinese Pharmaceutical Journal, 2009, 44(08): 580-584

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