Comparative Studies on Karyotypes of Gentiana dahurica Fisch and G.macrophylla Pall
LIU Li-sha，WANG Lan，SUN Shao-bo，WU Di
Author information+
Gansu College of Traditional Chinese Medicine，Lanzhou 730000，China
{{custom_zuoZheDiZhi}}
{{custom_authorNodes}}
{{custom_bio.content}}
{{custom_bio.content}}
{{custom_authorNodes}}
Collapse
History+
Received
Revised
Published
2008-04-19
2008-04-19
2008-04-19
Issue Date
2009-03-05
Abstract
OBJECTIVE To study karyotypes of Gentiana dahurica Fisch and G.macrophylla Pall for the first time.This will provide cytological evidence for further studies on genetics and evolution.METHODS The root tip was soaked in 0.002 mol·L-1 8-oxychinolin solution for 5.7 h.Then,the sample was decomposed in 2.5% mixed enzyme solution for 1.6 h at 25 ℃ and treated with Hypotonic for 3 h in refrigerator.At last,specimen slides were prepared by the air-drying technique.RESULTS The karyotypes formula of G.dahurica was K(2n)=2X=26=2M+24m,which belonged to "1A" and whose relative length of chromosomes was 2n=26=2L+10M2+12M1+2S.The karyotypes formula of G.macrophylla was K(2n)=2X=26=2M+20m+4sm,which belonged to "2B" and whose relative length of chromosomes was 2n=26=6L+6M2+8M1+6S.CONCLUSION Comparing karyotypes of G.dahurica with G.macrophylla,the result showed that G.macrophylla lives in higher stage of evolution than G.dahurica.
LIU Li-sh;WNG Ln;SUN Sho-o;WU Di.
Comparative Studies on Karyotypes of Gentiana dahurica Fisch and G.macrophylla Pall [J]. Chinese Pharmaceutical Journal, 2009, 44(05): 331-333
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] SUN S M. The Shennong Herb-Root Classic（神农本草经）[M]. Beijing：People's Medical Publishing House，1975：67.
[2] Ch.P（2005）Vol Ⅰ（中国药典2005版.一部）[S]. 2005：190-191.
[3] CHEN R Y，SONG W Q，LI X L. A new method for prepa-ring mitotic chromosomes from plant [J]. J Integratire Plant Biology（植物学报），1979，21(3)：297-298.
[4] LIU L S，ZHANG X L. Comparative studies on karyotypes of Cuscuta chinensis Lam. And C. japonica Choisy [J]. Chin Tradit Herb Drugs（中草药），2000，31(17)：784-786.
[5] LIU L S，JI K P. Germination characters of seed of Gentiana macrophylla [J]. Chin Tradit Herb Drugs（中草药），2002，33(3)：269-271.
[6] LEVEN A，FREDGA K，SANBERG A A. Nomenclature for centr-omeric position on chromosomes [J]. Hereditas，1964，52：201-220.
[7] KUO S R，WANG T T，HUANG T C. Karyotype Analysis of Some Formosan Gymnosperms [J]. Taiwania，1972，17(1)：66.
[8] STEBBINS G L. Chromosomal Evolution in Higher Plants[M]. London：Edward Arnold，1971：85-104.
[9] LI M X，CH R Y. A suggestion on the standardization of karyotype analysis in plants [J]. J Wuhan Bot Res（武汉植物学研究），1985，3(4)：297-301.
[10] ARANO H. Cytological studies in Subfamily carduoideae (Compositae) of Japan，IX. The karyotype analysis and phylog-enic considerations on Pertya and Anisliaea (2) [J]. Bot Mag (Tokyo)，1963，76：32-39.
[11] The Editrial Board of Flora China，Chinese Academy of Science. Flora of China（中国植物志）[M]. Vol 62. Beijing，Science Press，1988：59-75.
[12] AI T M. Medical Botany（药用植物学）[M]. Beijing：Peking University Press，2004：129-130.
