Deglycosylation of Earthworm Fibrinolytic Enzymes and Immunoreactivity Assay
WU Jin-xia，WU Ya-ping，ZHAO Xiao-yu，HAN Li-mei
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College of Life Science，Hebei University，Baoding 071002，China
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History+
Received
Published
2008-01-28
2008-12-10
Issue Date
2008-12-10
Abstract
OBJECTIVE To investigate the immunoreactivity of the deglycosylated EFEs.METHODS The antisera against earthworm fibrinolytic enzymes(EFEs) were developed by immunising rabbits with heat denatured EFEs.The oligosaccharides of EFEs and the component 6.5#,7# were removed by trifluoromethanesulfonate(TFMS) with the indication of SDS-PAGE and ConA-HRP affinity blotting.The methods of ELISA and Western-blotting were used to detect the differences of immunoreactivity between the samples and the deglycosylated ones.RESULTS There were no obvious changes of the immunoreactivity of EFEs and the component 6.5#,7# after being deglycosylated by TFMS.CONCLUSION The oligosaccharides of EFEs and the component 6.5#,7# show no influences on their immunoreactivities markedly.The oligosaccharides were not involved in the antigen determinants of EFEs.
WU Jin-xi;WU Y-ping;ZHO Xio-yu;HN Li-mei.
Deglycosylation of Earthworm Fibrinolytic Enzymes and Immunoreactivity Assay [J]. Chinese Pharmaceutical Journal, 2008, 43(24): 1848-1851
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