Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide
SUN Xiang-hong1，2，SUN Wei1，LI Jing2，GENG Mei-yu2
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1. Hospital Affiliated to Medical College，Qingdao University，Qingdao 266003，China； 2. School of Medicine and Pharmacy，Ocean University of China，Qingdao 266003，China
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History+
Received
Published
2007-12-21
2008-12-05
Issue Date
2008-12-05
Abstract
OBJECTIVE To observe the effect of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca2+ of neuroblastoma SH-SY5Y cells.METHODS The effects of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca2+ of SH-SY5Y cells impaired by hydogen peroxide(H2O2)cultured in vitro were measured by flow cytometry.RESULTS Compared with the model group,Haikangling obviously restrained the apoptosis,Ca2+) inflow,and stabilized the mitochondria membrane potential of SH-SY5Y cells.CONCLUSION Anti-dementia effects of Haikangling were related to restraining the apoptosis,stabilizing mitochondria membrane potential and restraining Ca2+ inflow of neurocyte.
SUN Xing-hong;SUN Wei;LI Jing;GENG Mei-yu.
Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide [J]. Chinese Pharmaceutical Journal, 2008, 43(23): 1781-1784
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References
[1] HUGON J, TERRO F, ESCLAIRE, at al. Markers of programmed cell death in Alzheimer's disease[J] . J Neural Transm Suppl，2000，59：125-131.
[2] ISHISAWA R，UTSUMI K，UTSUMI T. Involvement of lysos- omal cysteine proteasis in hydrogen peroxide-induced apoptosis in HL-60 cells[J] . Biosci Biotechnol Biochem，2002，66(9)：1865-1872.
[3] SUN X H，LIU H L，GEN M Y. Protective effects of the medical serum of Haikangling on the impairment of neuroblastoma SH-SY5Y cells cultured in vitro[J] . Chin Pharm J (中国药学杂志)，2006，41（10）：1465-1467.
[4] SLATER A F，STEFAN C，NOBEL I，et al. Intracellular redox changes during apoptosis[J] . Cell Death Differ，1996，3（1）：57-62.
[5] LENNON S V，MARTIN S J，COTTER T G. Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli[J] . Cell Prolif，1991，24(2)： 203-214.
[6] ISHISAKA R，UTSUMI K，UTSUMI T. Involvement of lysosomal cysteine proteases in hydrogen peroxide-induced apoptosis in HL-60 cells[J] . Biosci Biotechnol Biochem，2002，66(9)： 1865-1872.
[7] ZAMZAMI N，MARCHITTI P，CASTEDO M，et al. Reduction in mitochondrial potential constitutes an early irreversible step of progrommed lymphocyte death in vivo[J] . J Exp Med，1995，181(5)：1661-1672.
[8] KULMS D，SCHWARZ T. Independent contribution of three diff- erent pathways to ultraviolet-B-induced apoptosis[J] . Biochem Pharmacal，2002，64(5-6)：837-841.
[9] GREEN D，KROEMER S. The central executioners of apoptosis： caspase or mitochondrial[J] . Trends Cell Biol，1998，8(7)：267-271.
[10] TAGLIARINO C，PINK J J，DUBYAK G R，et al. Calcium is a key signaling molecule in beta-lapachone-mediated cell death[J] . J Biol Chem，2001，276 (22) ： 19150-19159.
[11] CANTONI O，SESTILI P，CATTABENI F，et al. Calcium chel- ator Quin 2 provents hydrogen-peroxide-induced DNA breakage and cytotoxicity[J] . Eur J Biochem，1989，182(2)：209-212.
[12] GUO Q，FU W，XIE J，et al. Par-4 is a mediator of neuronal degeneration associated with the pathogenesis of Alzheimer's disease[J] . Net Med，1998，4(8)：957-962.
