Abstract
OBJECTIVE To establish an identification and quantification method in fermentation by GC.METHODS The trichodermin was determined by GC with HP-5 capillary column(5% phenyl methyl siloxane,30 m×320 μm,0.25 μm) with FID detector and nitrogen as the carries gas.The injector temperature was 250 ℃,the detector temperature was 270 ℃,the column temperature was increased by program,the initial temperature was 100 ℃,maintained for 5 min,raised to 260 ℃ with a rate of 10 ℃·min-1,maintained for 5 min.The flow rate of N2 was 1.5 mL·min-1.RESULTS Trichodermin was completely separated by GC,the linearity was in the range of(0.01～0.5) g·L-1,average recovery was 99.76%,RSD was 1.69%.CONCLUSION The method of GC was accurate,simple,rapid and producible.
Key words
GC /
trichodermin /
test methods
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TU Xu-ping;SHI Yi-jun;YU Xio-ping.
Identification and Quantification of Trichodermin in Fermentation by GC [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1755-1756
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References
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