OBJECTIVE To develop a high performance liquid chromatography method for the simultaneous determination of five major lignans(schisandrin,gomisin A,angeloylgomisin H,deoxyschizandrin and γ-schisandrin) in Tangke capsule.METHODS Chromatographic separation was performed on an Agilent Extend C18 column.The mobile phase was composed of methanol and 2% tetrahydrofuran-water at a linear gradient elution.The column temperature was maintained at 50 ℃.Detection wavelength was 254 nm,and flow rate was 0.8 mL·min-1.RESULTS Good linear relationships of five lignans were provided over investigated concentration ranges.The linear ranges of schisandrin,gomisin A,angeloylgomisin H,deoxyschizandrin and γ-schisandrin were 15.8～1 575,5.76～576,4.65～465,3.61～361,10.6～1 057 mg·L-1,respectively.The values of correlation coefficient were higher than 0.999 for all the analytes.The average recoveries of the five components ranged from 100.2% to 103.5%.Repeatability experiments showed that relative standard deviation(RSD) values of their contents were less than 1.98%.CONCLUSION The developed method is simple,sensitive and repeatable.The method can be used for quality control of Tangke capsule.
CHEN Ying-jie;SHO Qing;QU Hi-in.
Simultaneous Determination of Five Lignan Compounds in Tangke Capsules by HPLC [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1751-1754
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