Study on in Situ Single Pass Perfusion Intestinal Absorption of Columbianetin Acetate

WU Y-n;LUN Li-io

Chinese Pharmaceutical Journal ›› 2008, Vol. 43 ›› Issue (22) : 1719-1722.

Chinese Pharmaceutical Journal ›› 2008, Vol. 43 ›› Issue (22) : 1719-1722.
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Study on in Situ Single Pass Perfusion Intestinal Absorption of Columbianetin Acetate

  • WU Ya-na，LUAN Li-biao
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Abstract

OBJECTIVE To study the intestinal absorption behavior of columbianetin acetate.METHODS Single pass perfusion model was used to study the absorption of columbianetin acetate at different drug concentrations and in various pH perfusion solutions respectively.The concentration of columbianetin acetate was determined by HPLC.RESULTS The absorption rate constants(Ka) and apparent permeability coefficients(Kapp) of columbianetin acetate were sequenced as follows colon > ileum > duodenum> jejunum in four different regions of rat intestine.The Ka and Kapp of colon and ileum were significant different from those of duodenum and jejunum(P<0.05).No difference(P>0.05) in Ka and Kapp was observed in the concentration ranges of 61.93～165.14 mg·L-1 and in pH ranges of 3～5.CONCLUSION Columbianetin acetate can be absorbed in whole intestinal sections and colon is the best absorption region of whole rat intestines.The increase of columbianetin acetate concentration has no effect on absorption kinetics,the absorption of columbianetin acetate is a passive diffusion process.The absorption of the drug is not pH-dependent.

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columbianetin acetate / absorption / in situ single pass perfusion model / HPLC

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WU Y-n;LUN Li-io. Study on in Situ Single Pass Perfusion Intestinal Absorption of Columbianetin Acetate [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1719-1722

References

[1] SCHURGERS N，BIJDENDIJK J，TUKKER J J， et al. Compa- rison of four exermental techniques for studying drug absorption kinetics in anesthetized rat in situ[J] . J Pharm Sci，1986，75 (2)：117-119. [2] FAGERHOLM U，JOHANSSON M，LENNERNAS H. Compa- rison between permeability coefficients in rat and human jejunum[J] . Pharm Res，1996，13 (9)：336-342. [3] PASCAL L C，GILES D，FRANCOIS C， et al. Influence of hydroxypropyl-β-cyclodextrin and dimethyl-β-cyclodextrin on diphenhydramine intestinal absorption in a rat in situ model [J] . Int J Pharm，1998，169：221-228. [4] LIAO Z G，PING Q N，XIAO W， et al. The study in situ on rat intestinal absorption of the active components in Guizhi Fuling Capsule[J] . Chin J Nat Med(中国天然药物)， 2005，3(5)：303-307. [5] CHEN W M，YAO X M，ZHANG H Q. Determination of two coumarin contents in the root of Angelica biserrata by HPLC method[J] . J Plant Resour Environ(植物资源与环境学报)， 2000，9(4)：51-52. [6] SUTTON C S， RINALDI T S， VUKOVINSKY K E， et al. Comparison of the gravimetric， phenol red， and 14C-PEG-3350 methods to determine water absorption in the rat single-pass intestinal perfusion model[J] . AAPS Pharm Sci，2001，3(1)：25. [7] YOU J， LI Q B， YU Y W， et al. Absorption of zedoary oil in rat intestine using in situ single pass perfusion model[J] . Acta Pharm Sin(药学学报)， 2004， 39(10)：849-853.

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