1.Medical School of Xi′an Jiaotong University，Xi′an 710061，China；2.Xi′an Medical College，Xi′an 7210021，China；3.Department of Pharmacy，Xi′an Jiaotong University，Xi′an 710061，China
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History+
Received
Published
2007-11-08
2008-11-10
Issue Date
2008-11-10
Abstract
OBJECTIVE To invetigate the chromatography affinities of subtype-selective α1D-adrenergic receptor(AR)antagonist(BMY7378) to rat aorta tissue cells,primary cultured rat aorta smooth muscle cell and α1D-AR subtype for improving the selectivity and specificity of CMC.METHODS According to predominant α1D-AR express in rat aorta,the homogenized rat aorta tissue cells chromatographic column,primary cultured rat aorta smooth muscle cell chromatographic column and α1D-AR subtypes over-expressed in the human embryonic kidney(HEK293) cell lines chromatographic column were designed and constructed.Six different concentrations of the BMY7378 were available in CMC experiments.Capacity factors(k′)of different concentrations of BMY7378,as a chromatographic parameter,were investigated and calculated.RESULTS With the preparation of CMSP of rat aorta tissue,the lowest concentration of BMY7378 was 1.00 mmol·L-1.With CMSP preparing of primary cultured rat aorta smooth muscle cell,the lowest concentration of BMY7378 was 0.03 mmol·L-1.The lowest concentration of BMY7378 with α1D-AR subtype obtained from CMC was 0.10 mmol·L-1.CONCLUSION Compared with traditional CMC method,the new method will further improve sensitivity and specificity of CMC and can be used to screen receptor-subtype drugs.
ZHNG Din;YUN ing-xing;DENG Xiu-ling;YNG Gung-de;HE Lng-chong.
Comparative Studies on Cell Membrane Chromatography with Rat Aorta Tissue,Cultured Rat Aorta Smooth Muscle Cell and α1D Subtypes Over Expressed Cell [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1710-1713
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