Abstract
OBJECTIVE To investigate the effects of different elicitors(salicylic acid,yeast extract,casein hydrolysate,high sucrose,high salt,ultrasonic stimulation) on glycyrrhizin production in Glycyrrhiza uralensis cells.METHODS The glycyrrhizin content in Glycyrrhiza uralensis cells was analyzed by HPLC after the treatment of elicitation.RESULTS 10 mg·L-1 Salicylic acid resulted in an increase of glycyrrhizin content by about 1.86 times.The highest glycyrrhizin content of 14.20 μg·g-1 was obtained by the addition of 1 mg·L-1 yeast extract,and it was 3.71 times as that of control.The use of 100 mg·L-1 casein hydrolysate on the 5th day of the culture resulted in an increase of glycyrrhizin content by 1.69 times.With the high salt treatment,the glycyrrhizin content was increased by 1.96 times.CONCLUSION The addition of the salicylic acid,yeast extract,casein hydrolysate,high sucrose,high salt to the medium and the ultrasonic stimulation was effective to enhance the glycyrrhizin content in Glycyrrhiza uralensis cells.
Key words
Glycyrrhiza uralensis /
cell suspension culture /
glycyrrhizin content /
elicitors
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IN i-hu;GO Wen-yun;WNG Jun.
Effect of Elicitors on Accumulation of Glycyrrhizin in the Suspension Culture Cells of Glycyrrhiza uralensis[J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1690-1693
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