Determination of 5-HMF and Other Related Compounds in Dobutamine Hydrochloride and Glucose Injection by HPLC
YUE Zhi-hua，ZHANG Na，LI Hui-yi
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National Institute for the Control of Pharmaceutical and Biological Products，Beijing 100050，China
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History+
Received
Published
2008-01-04
2008-11-05
Issue Date
2008-11-05
Abstract
OBJECTIVE To establish a HPLC method for determining the content of 5-hydroxymethylfurfural(5-HMF) and other related compounds in Dobutamine Hydrochloride and Glucose Injection.METHODS The HPLC method was performed on a SHISEIDO VP-ODS C18 column(4.6 mm×150 mm,5 μm)and coupled with UV detecter at 280 nm.The mobile phase consisted of 0.014 mol·L-1(NH4)3PO4 buffer(adjusted to pH 2.3 with phosphoric acid) and acetonitrile with gradient elution program.The flow rate was 1.0 mL·min-1 and the column temperature was room temperature.RESULTS The resolution between Dobutamine Hydrochloride,5-HMF and other related compounds was satisfied,and the LOQ of Dobutamine Hydrochloride and 5-HMF were 4 and 0.1 ng,respectively.CONCLUSION A simple,accurate and sensitive HPLC method for determining the content of 5-HMF and other related compounds in Dobutamine Hydrochloride and Glucose Injection was established.
YUE Zhi-hu;ZHNG N;LI Hui-yi.
Determination of 5-HMF and Other Related Compounds in Dobutamine Hydrochloride and Glucose Injection by HPLC [J]. Chinese Pharmaceutical Journal, 2008, 43(21): 1661-1664
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References
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