Determination of Piperacillin/Tazobactam in Human Plasma and Urine by High-Performance Liquid Chromatography

SUN Y-xin;ZHO Li-mei;HE Xio-jing;QIU Feng

Chinese Pharmaceutical Journal ›› 2008, Vol. 43 ›› Issue (21) : 1651-1653.

Chinese Pharmaceutical Journal ›› 2008, Vol. 43 ›› Issue (21) : 1651-1653.
论著

Determination of Piperacillin/Tazobactam in Human Plasma and Urine by High-Performance Liquid Chromatography

  • SUN Ya-xin，ZHAO Li-mei*，HE Xiao-jing，QIU Feng
Author information +
History +

Abstract

OBJECTIVE To develop and validate a simple and rapid high-performance liquid chromatographic(HPLC) method for the simultaneous determination of piperacillin/tazobactam in human plasma and urine.METHODS Plasma samples were deproteinized with acetonitrile followed by extraction of endogeous compounds with dichloromethane.The upper aquous phase and diluted urine samples with mobile phase were injected into HPLC for determination.The analysis was conducted on a CAPCELL PAK C18 column with gradient elution,the detecting wavelengh was at 220 nm.RESULTS The method was linear in the range from 0.5 to 400 mg·L-1 for piperacillin and from 0.5 to 100 mg·L-1 for tazobactam.The linear range of piperacillin/tazobactam in human urine were 2.5 to 2 000 mg·L-1 for piperacillin and 2.5 to 500 mg·L-1 for tazobactam.The absolute recoveries were 85.3%-92.9% for piperacillin and 87.6%-90.8% for tazobactam at three different concentrations.The intra-and inter-day precisions of the method(RSD) were lower than 10% for both compounds.CONCLUSION This method is suitable for the pharmacokinetic study of piperacillin/tazobactam.

Key words

piperacillin / tazobactam / HPLC / pharmacokinetics

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
SUN Y-xin;ZHO Li-mei;HE Xio-jing;QIU Feng. Determination of Piperacillin/Tazobactam in Human Plasma and Urine by High-Performance Liquid Chromatography [J]. Chinese Pharmaceutical Journal, 2008, 43(21): 1651-1653

References

[1] TRITTLER R，EHRLICH M，GALLA T J，et al. New and rapid fully automated method for determination of tazobactam and piperacillin in fatty tissue and serum by column-switching liquid chromatography[J] .J Chromatogr B Analyt Technol Biomed Life Sci，2002，775(2)：127-132. [2] OCAMPO A P，HOYT K D，WADGAONKAR N，et al. Deter mination of tazobactam and piperacillin in human plasma，serum，bile and urine by gradient elution reversed-phase high-performance liquid chromatography[J] .J Chromatogr，1989，496(1)：167-179. [3] HUANG Z Y，TANG Z，QIU Y Q，et al. Pharmacokinetic study of two piperacillin sodium/tazobactam preparations in Chinese healthy volunteers[J] .Chin J Clin Pharm(中国临床药学杂志)，2000，9(2)：78-81. [4] SORGEL F，KINZIG M. Pharmacokinetics and tissue penetration of piperacillin/tazobactam with particular reference to its potential in abdominal and soft tissue infections[J] .Eur J Surg Suppl，1994，573：39-44. [5] KEES F，NABER K G，BARTOSCHIK-WICH B，et al. High-performance liquid chromatography analysis of mezlocillin，piperacillin，their degradation products，and of ioxitalamic acid in plasma and urine of healthy volunteers[J] . Arzneimittelforschung，1985，35(7)：1099-1104.

Accesses

Citation

Detail
Sections
Recommended

/

〈 〉