Study on Preparation and in Vitro Release Behavior of Lung Targeting Hydroxycamptothecin Liposomes
SONG Jin-chun1，HUANG Ling2，CHEN Jia-li2
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1.Department of Pharmacy，Renmin Hospital of Wuhan University，Wuhan 430060，China；2.College of Pharmacy，Wuhan University，Wuhan 430072，China
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History+
Received
Published
2007-11-09
2008-10-10
Issue Date
2008-10-10
Abstract
OBJECTIVE To prepare lung targeting hydroxycamptothecin(HCPT) cationic liposomes and study the release of HCPT in vitro.METHODS The hydroxycamptothecin liposomes were prepared by thin film method with freeze-thawing steps.D-mannose was used to coat the liposomes for lung targeting action and lipid membrane was modified with stearylamine.Unloaded hydroxycamptothecin were separated by sephadex G50 chromatography.HPLC method was used to determine the entrapment efficiency of hydroxycamptothecin liposomes.Orthogonal design was used to select the optimum formulation.Dialysis was used to study the release of HCPT in vitro.RESULTS The prepared hydroxycamptothecin liposomes demonstrated good stability and encapsulation efficiency was more than 65%.The average size was 2.286 μm and the Zeta potential of the modified hydroxycamptothecin liposomes was +21.5 mV.The release in vitro was characterized by Higuchi equation.CONCLUSION Thin film method with freeze-thawing steps could increase the entrapment efficiency and stability of hydroxycamptothecin liposomes after modification of lipid membrane with D-mannose and stearylamine.It was valuable to be further studied for lung target.
SONG Jin-chun;HUNG Ling;CHEN Ji-li.
Study on Preparation and in Vitro Release Behavior of Lung Targeting Hydroxycamptothecin Liposomes [J]. Chinese Pharmaceutical Journal, 2008, 43(20): 1564-1567
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References
[1] ZHOU J J，LIU J，XU B. Relationship between lactone ring forms of HCPT and their antitumor activities[J] . Acta Pharmacol Sin(中国药理学报)，2001，22(9)：827-830.
[2] WU Y，HE W，DAI W B，et al. Study on formulaion and preparation of chitosan chloride coated hydroxycamptothecin nanoliposomes[J] .Chin Pharm J（中国药学杂志），2005，40(12)：922-925.
[3] BI D Z. Pharmaceutics(药剂学)[M] .4th Ed. Beijing：People′s Health Publishing House，2000：449-450.
[4] KELLAWAY I W，FARR S J. Liposoms as drug delivery systems to the lung[J] .Adv Drug Dliv Rev，1990，5(1)：149-161.
[5] MONNARD P A，OBERHOLZER T，LUISI P. Entrapment of nucleic acids in liposomes[J] .Biochin Biophys Acta，1997，1329（1）：39-50.
[6] FASSBERG J，STELLA V J.A kinetic and mechanistic study of the hydrolysis of camptothecin and some analogues[J] .J Pharm Sci，1992，81(7)：676-684.
[7] LAURSEN S B，THIEL S，TEISNER B. Bovine conglutinin binds to an oligo saccharide determinant presented by iC3b，but not by C3，C3b or C3c[J] .Immunology，1994，81(4)：648-654.
